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Functional expression of the Fc-fused extracellular domains of group II membrane proteins
- 作者:Weicheng Wu (1)
Jing Jin (3) Xiaojuan Liu (1) (6) Yufei Zhang (4) Meng Li (5) Miaomiao Shao (1) Yifan Qian (1) Dongmei Zhang (1) Haiyan Zhu (1) Yuanyuan Ruan (1) Jianhui Xie (1) (2) Jianxin Gu (1)
1. Key Laboratory of Glycoconjugate Research ; Ministry of Health ; Department of Biochemistry and Molecular Biology ; Shanghai Medical College ; Fudan University ; Shanghai ; 200032 ; China 3. Institute of Glycobiological Engineering ; Zhejiang Provincial Key Laboratory of Medical Genetics ; Wenzhou Medical College ; Wenzhou ; 325035 ; China 6. Department of Pathogen Biology ; Medical College ; Nantong University ; Nantong ; Jiangsu ; 226001 ; China 4. Department of Immunology ; Shanghai Medical College ; Fudan University ; Shanghai ; 200032 ; China 5. Department of Dermatology ; Shanghai Key Laboratory of Molecular ; Mycology & PLA Key Laboratory of Fungal Diseases ; Changzheng Hospital ; Second Military Medical University ; Shanghai ; 200032 ; China 2. Department of Forensic Medicine ; Shanghai Medical College ; Fudan University ; Shanghai ; 200032 ; China
- 关键词:Fc moiety ; Extracellular domain ; Group II membrane proteins ; CLEC ; 2
- 刊名:Glycoconjugate Journal
- 出版年:2015
- 出版时间:February 2015
- 年:2015
- 卷:32
- 期:1-2
- 页码:69-76
- 全文大小:2,132 KB
- 参考文献:1. Wickner, WT, Lodish, HF (1985) Multiple mechanisms of protein insertion into and across membranes. Science 230: pp. 400-407 CrossRef
2. Velu, TJ (1990) Structure, function and transforming potential of the epidermal growth factor receptor. Mol. Cell. Endocrinol. 70: pp. 205-216 CrossRef 3. Schneider, C, Asser, U, Sutherland, DR, Greaves, MF (1983) In vitro biosynthesis of the human cell surface receptor for transferrin. FEBS Lett. 158: pp. 259-264 CrossRef 4. Ohtsubo, K, Marth, JD (2006) Glycosylation in cellular mechanisms of health and disease. Cell 126: pp. 855-867 CrossRef 5. Young, CL, Britton, ZT, Robinson, AS (2012) Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications. Biotechnol. J. 7: pp. 620-634 CrossRef 6. Durocher, Y, Butler, M (2009) Expression systems for therapeutic glycoprotein production. Curr. Opin. Biotechnol. 20: pp. 700-707 CrossRef 7. Waugh, DS (2005) Making the most of affinity tags. Trends Biotechnol. 23: pp. 316-320 CrossRef 8. Cannon, JP, O鈥橠riscoll, M, Litman, GW (2011) Construction, expression, and purification of chimeric protein reagents based on immunoglobulin fc regions. Methods Mol. Biol. 748: pp. 51-67 CrossRef 9. Figdor, CG, Kooyk, Y, Adema, GJ (2002) C-type lectin receptors on dendritic cells and Langerhans cells. Nat. Rev. Immunol. 2: pp. 77-84 CrossRef 10. Robinson, MJ, Sancho, D, Slack, EC, LeibundGut-Landmann, S (2006) Reis e Sousa, C.: Myeloid C-type lectins in innate immunity. Nat. Immunol. 7: pp. 1258-1265 CrossRef 11. Suzuki-Inoue, K, Inoue, O, Ozaki, Y (2011) Novel platelet activation receptor CLEC-2: from discovery to prospects. J. Thromb. Haemost. 9: pp. 44-55 CrossRef 12. Dunn, S, Vohra, RS, Murphy, JE, Homer-Vanniasinkam, S, Walker, JH, Ponnambalam, S (2008) The lectin-like oxidized low-density-lipoprotein receptor: a pro-inflammatory factor in vascular disease. Biochem. J. 409: pp. 349-355 CrossRef 13. Brown, GD (2006) Dectin-1: a signalling non-TLR pattern-recognition receptor. Nat. Rev. Immunol. 6: pp. 33-43 CrossRef 14. Zhu, H, Wang, L, Ruan, Y, Zhou, L, Zhang, D, Min, Z, Xie, J, Yu, M, Gu, J (2011) An efficient delivery of DAMPs on the cell surface by the unconventional secretion pathway. Biochem. Biophys. Res. Commun. 404: pp. 790-795 CrossRef 15. Kooyk, Y, Rabinovich, GA (2008) Protein-glycan interactions in the control of innate and adaptive immune responses. Nat. Immunol. 9: pp. 593-601 CrossRef 16. Schwarz, F, Aebi, M (2011) Mechanisms and principles of N-linked protein glycosylation. Curr. Opin. Struct. Biol. 21: pp. 576-582 CrossRef 17. Hossler, P, Khattak, SF, Li, ZJ (2009) Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology 19: pp. 936-949 CrossRef 18. Watson, AA, Brown, J, Harlos, K, Eble, JA, Walter, TS, O鈥機allaghan, CA (2007) The crystal structure and mutational binding analysis of the extracellular domain of the platelet-activating receptor CLEC-2. J. Biol. Chem. 282: pp. 3165-3172 CrossRef 19. Suzuki, T, Ishii-Watabe, A, Tada, M, Kobayashi, T, Kanayasu-Toyoda, T, Kawanishi, T, Yamaguchi, T (2010) Importance of neonatal FcR in regulating the serum half-life of therapeutic proteins containing the Fc domain of human IgG1: a comparative study of the affinity of monoclonal antibodies and Fc-fusion proteins to human neonatal FcR. J. Immunol. 184: pp. 1968-1976 CrossRef
- 刊物类别:Biomedical and Life Sciences
- 刊物主题:Life Sciences
Biochemistry Pathology
- 出版者:Springer Netherlands
- ISSN:1573-4986
文摘
The complicated delivery mechanism of group II membrane proteins makes it difficult to decide the fusion pattern of their extracellular domains (ECDs) with Fc moiety. In this study, we compared the expression of ECDs of three group II membrane proteins including CLEC-2, Dectin-1, and LOX-1 by fusion of Fc moiety. We found that the pattern of ECD-Fc fusion order produced the functionally active recombinant proteins while the pattern of Fc-ECD fusion order led to the altered glycosylation which abolished the binding of these proteins with their ligands. Meanwhile, our results indicated that the secretion of mouse Fc (mFc)-fused ECD of CLEC-2 was more efficient than that of rabbit Fc (rFc)-fused protein, while rFc moiety was more sensitive for detection compared with mFc moiety. Altogether, we provide a favorable fusion pattern of Fc moiety with the ECDs of group II transmembrane proteins.
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