Study of the upregulation of the activity of cytochrome P450 3A isoforms by Astragalus injection and Astragalus granules in rats and in cells

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• 作者：Yongli Zhang (1) (2)
  Ling Huang (3)
  Huichang Bi (3)
  Yuqiang Cui (1) (2)
  Jingqing Li (3)
  Xiangsheng Wang (1) (2)
  Xiaoling Qin (3)
  Jiangying Chen (3)
  Min Huang (3)
  
• 关键词：Astragalus injection (AI) ; Astragalus granules (AG) ; Cytochrome P450 3A (CYP3A) ; CYP3A1 isoform ; CYP3A4 isoform ; Induction
• 刊名：European Journal of Drug Metabolism and Pharmacokinetics
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：38
• 期：2
• 页码：105-113
• 全文大小：384KB
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• 作者单位：Yongli Zhang (1) (2)
  Ling Huang (3)
  Huichang Bi (3)
  Yuqiang Cui (1) (2)
  Jingqing Li (3)
  Xiangsheng Wang (1) (2)
  Xiaoling Qin (3)
  Jiangying Chen (3)
  Min Huang (3)
  
  1. Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, 280 Wai Huan Dong Road, University City of Guangzhou, Guangzhou, 510006, Guangdong, People’s Republic of China
  2. Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, Guangdong, People’s Republic of China
  3. Lab of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China
  

文摘

Astragalus injection (AI) and Astragalus granules (AG) are the two representative clinical preparations from Astragali Radix. In order to investigate the regulation of metabolism, AI and AG were tested for their ability to affect the major enzyme cytochrome P450 3A isoforms in vivo and in vitro. In the study of CYP3A1 enzyme activity, male rats were pretreated with AI and AG. The “cocktail-approach-based LC–MS/MS results showed that AI pretreatment at 0.16, 0.8 and 4?g?kg??day? significantly increased the rat liver microsome CYP3A1 activity by 1.62-, 1.68- and 2.00-fold, and AG pretreatment at 32, 160 and 800?mg?kg??day? significantly increased the rat CYP3A1 activity by 1.86-, 2.16- and 1.76-fold. The effects of AI and AG on liver microsome CYP3A1 mRNA expression in rats were analyzed using real-time PCR technique. The results showed that AI and AG pretreatments significantly increased the CYP3A1 mRNA expression. The induction of CYP3A4 enzyme activity by AI and AG in vitro was measured using a CYP3A4 luciferase reporter gene assay in transiently transfected human intestinal LS174T cells. Compared to the control group, AI at 62.5-,000?mg/ml could significantly induce CYP3A4 reporter gene luciferase activity of 1.36- to 1.88-fold for 48-h incubated PXR-transfected LS174T cells, and AG at 62.5-,000?μg/ml significantly transactivated CYP3A4 reporter gene luciferase activity of 1.36- to 2.05-fold. However, the CYP3A4 reporter gene construct was not significantly transactivated by the AI and AG in CAR-transfected LS174T cells. These CYP3A isoforms upregulation results can help us to use AI and AG rationally in the clinic.