Cripto-1 modulates macrophage cytokine secretion and phagocytic activity via NF-κB signaling

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• 作者：Dong-mei Zhang ; Yong-Li Bao ; Chun-Lei Yu ; Yi-meng Wang…
• 关键词：Cripto ; 1 protein ; Immunity ; Macrophages ; Nuclear factor kappa ; B ; Tumor
• 刊名：Immunologic Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：64
• 期：1
• 页码：104-114
• 全文大小：7,313 KB
• 参考文献：1.Strizzi L, et al. Emerging roles of nodal and Cripto-1: from embryogenesis to breast cancer progression. Breast Dis. 2008;29:91–103.PubMedCentral PubMed
  2.Salomon DS, Bianco C, De Santis M. Cripto: a novel epidermal growth factor (EGF)-related peptide in mammary gland development and neoplasia. BioEssays. 1999;21(1):61–70.CrossRef PubMed
  3.D’Andrea D, et al. Cripto promotes A–P axis specification independently of its stimulatory effect on Nodal autoinduction. J Cell Biol. 2008;180(3):597–605.PubMedCentral CrossRef PubMed
  4.Bianco C, et al. Regulation of Cripto-1 signaling and biological activity by caveolin-1 in mammary epithelial cells. Am J Pathol. 2008;172(2):345–57.PubMedCentral CrossRef PubMed
  5.Wu Z, et al. Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma. BMC Cancer. 2009;9:315.PubMedCentral CrossRef PubMed
  6.Yeo C, Whitman M. Nodal signals to Smads through Cripto-dependent and Cripto-independent mechanisms. Mol Cell. 2001;7(5):949–57.CrossRef PubMed
  7.Gray PC, et al. Cripto binds transforming growth factor beta (TGF-beta) and inhibits TGF-beta signaling. Mol Cell Biol. 2006;26(24):9268–78.PubMedCentral CrossRef PubMed
  8.De Santis ML, et al. Cripto-1 inhibits beta-casein expression in mammary epithelial cells through a p21ras-and phosphatidylinositol 3′-kinase-dependent pathway. Cell Growth Differ. 1997;8(12):1257–66.PubMed
  9.Dunn GP, et al. Cancer immunoediting: from immunosurveillance to tumor escape. Nat Immunol. 2002;3(11):991–8.CrossRef PubMed
  10.Dunn GP, Old LJ, Schreiber RD. The three Es of cancer immunoediting. Annu Rev Immunol. 2004;22:329–60.CrossRef PubMed
  11.Johnston FM, et al. Circulating mesothelin protein and cellular antimesothelin immunity in patients with pancreatic cancer. Clin Cancer Res. 2009;15(21):6511–8.PubMedCentral CrossRef PubMed
  12.Siveen KS, Kuttan G. Role of macrophages in tumour progression. Immunol Lett. 2009;123(2):97–102.CrossRef PubMed
  13.Hagemann T, et al. Macrophages induce invasiveness of epithelial cancer cells via NF-kappa B and JNK. J Immunol. 2005;175(2):1197–205.CrossRef PubMed
  14.Deng L, et al. A novel mouse model of inflammatory bowel disease links mammalian target of rapamycin-dependent hyperproliferation of colonic epithelium to inflammation-associated tumorigenesis. Am J Pathol. 2010;176(2):952–67.PubMedCentral CrossRef PubMed
  15.Lin EY, et al. Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages. Mol Oncol. 2007;1(3):288–302.PubMedCentral CrossRef PubMed
  16.Torroella-Kouri M, et al. Identification of a subpopulation of macrophages in mammary tumor-bearing mice that are neither M1 nor M2 and are less differentiated. Cancer Res. 2009;69(11):4800–9.CrossRef PubMed
  17.Bolpetti A, et al. Interleukin-10 production by tumor infiltrating macrophages plays a role in human papillomavirus 16 tumor growth. BMC Immunol. 2010. doi:10.​1186/​1471-2172-11-27 .PubMedCentral PubMed
  18.Hao NB, et al. Macrophages in tumor microenvironments and the progression of tumors. Clin Dev Immunol. 2012;2012:948098. doi:10.​1155/​2012/​948098 .
  19.Minchiotti G, et al. Membrane-anchorage of Cripto protein by glycosylphosphatidylinositol and its distribution during early mouse development. Mech Dev. 2000;90(2):133–42.CrossRef PubMed
  20.Grivennikov SI, Greten FR, Karin M. Immunity, inflammation, and cancer. Cell. 2010;140(6):883–99.PubMedCentral CrossRef PubMed
  21.Gocheva V, et al. IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion. Genes Dev. 2010;24(3):241–55.PubMedCentral CrossRef PubMed
  22.Galli SJ, Borregaard N, Wynn TA. Phenotypic and functional plasticity of cells of innate immunity: macrophages, mast cells and neutrophils. Nat Immunol. 2011;12(11):1035–44.PubMedCentral CrossRef PubMed
  23.Schommer NN, et al. Staphylococcus epidermidis uses distinct mechanisms of biofilm formation to interfere with phagocytosis and activation of mouse macrophage-like cells 774A.1. Infect Immun. 2011;79(6):2267–76.PubMedCentral CrossRef PubMed
  
• 作者单位：Dong-mei Zhang (1) (2)
  Yong-Li Bao (1)
  Chun-Lei Yu (3)
  Yi-meng Wang (1)
  Zhen-Bo Song (3)
  
  1. National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China
  2. Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education, Northeast Normal University, Changchun, 130024, China
  3. Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024, China
  
• 刊物主题：Allergology; Immunology; Medicine/Public Health, general; Internal Medicine;
• 出版者：Springer US
• ISSN：1559-0755

文摘

Cripto-1 is an oncogenic protein belonging to the epidermal growth factor–Cripto-1/FRL-1/Cryptic family. It has important roles in tumor formation and metastasis, but its effects on the immune system are unclear. In the present study, we investigated the effects of Cripto-1 overexpression on macrophage activities and examined the underlying mechanisms. A cell line stably overexpressing Cripto-1 was developed. The culture supernatant from this cell line was collected and used to condition macrophages (RAW264.7, THP-1, and primary mouse macrophages) for various times. Exposure to this supernatant significantly increased the mRNA and protein expression levels of the anti-inflammatory cytokine interleukin (IL)-10 and of three pro-inflammatory cytokines (tumor necrosis factor-α, IL-6, and IL-1β), but did not affect the expression of transforming growth factor-β, another anti-inflammatory cytokine. Exposure to this supernatant also enhanced macrophage phagocytosis of chicken erythrocytes and yeast cells. Similar effects were observed in macrophages stimulated with purified Cripto-1 protein. Mechanistic experiments revealed that Cripto-1 activated nuclear factor (NF)-κB signaling by inducing IκB kinase phosphorylation and p65 nuclear translocation. Pretreatment with ammonium pyrrolidine dithiocarbamate, a specific NF-κB inhibitor, inhibited Cripto-1-induced cytokine secretion and phagocytosis of macrophages. Taken together, our present findings suggest that Cripto-1 enhances macrophage phagocytic activity and upregulates the production of anti- and pro-inflammatory cytokines via the NF-κB signaling pathway. Keywords Cripto-1 protein Immunity Macrophages Nuclear factor kappa-B Tumor