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Clinical significance of the uPA system in gastric cancer with peritoneal metastasis
- 作者：Youcheng Ding (1)
Hui Zhang (1)
Mingan Zhong (1)
Zhuqing Zhou (1)
Zhixiang Zhuang (1)
Hua Yin (1)
Xujing Wang (1)
Zhenggang Zhu (2)
- 关键词：Gastric cancer ; ELISA ; Peritoneal metastasis ; RT ; PCR ; UPA system
- 刊名：European Journal of Medical Research
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：18
- 期：1
- 全文大小：218KB
- 参考文献：1. Yang L: Incidence and mortality of gastric cancer in China. / World J Gastroenterol 2006, 12:17.
2. Yoo C, Noh S, Shin D, Choi S, Min J: Recurrence following curative resection for gastric carcinoma. / British J Surg 2000, 87:236鈥?42. CrossRef
3. Wei Y, Tang CH, Kim Y, Robillard L, Zhang F, Kugler MC, Chapman HA: Urokinase receptors are required for 伪5尾1 integrin-mediated signaling in tumor cells. / J Biol Chem 2007, 282:3929. CrossRef
4. Lee DH, Yang Y, Lee SJ, Kim KY, Koo TH, Shin SM, Song KS, Lee YH, Kim YJ, Lee JJ: Macrophage inhibitory cytokine-1 induces the invasiveness of gastric cancer cells by up-regulating the urokinase-type plasminogen activator system. / Cancer Res 2003, 63:4648.
5. de Bock CE, Wang Y: Clinical significance of urokinase鈥抰ype plasminogen activator receptor (uPAR) expression in cancer. / Med Res Rev 2004, 24:13鈥?9. CrossRef
6. Dass K, Ahmad A, Azmi AS, Sarkar SH, Sarkar FH: Evolving role of uPA/uPAR system in human cancers. / Canc Treat Rev 2008, 34:122鈥?36. CrossRef
7. Kwaan HC, McMahon B: The role of plasminogen-plasmin system in cancer. / Cancer Treat Res 2009, 148:43鈥?6. CrossRef
8. Andreasen PA, Kj酶ller L, Christensen L, Duffy MJ: The urokinase鈥抰ype plasminogen activator system in cancer metastasis: a review. / Int J Cancer 1997, 72:1鈥?2. CrossRef
9. Liotta LA, Kohn EC: The microenvironment of the tumour-host interface. / Nature 2001, 411:375鈥?79. CrossRef
10. Plebani M, Hersz猫nyi L, Carraro P, De Paoli M, Roveroni G, Cardin R, Tulassay Z, Naccarato R, Farinati F: Urokinase-type plasminogen activator receptor in gastric cancer: tissue expression and prognostic role. / Clin Exp Metastasis 1997, 15:418鈥?26. CrossRef
11. Taniguchi K, Yonemura Y, Nojima N, Hirono Y, Fushida S, Fujimura T, Miwa K, Endo Y, Yamamoto H, Watanabe H: The relation between the growth patterns of gastric carcinoma and the expression of hepatocyte growth factor receptor (c鈥抦et), autocrine motility factor receptor, and urokinase鈥抰ype plasminogen activator receptor. / Cancer 1998, 82:2112鈥?122. CrossRef
12. Lee KH, Bae SH, Lee JL, Hyun MS, Kim SH, Song SK, Kim HS: Relationship between urokinase-type plasminogen receptor, interleukin-8 gene expression and clinicopathological features in gastric cancer. / Oncology 2004, 66:210鈥?17. CrossRef
13. Kaneko T, Konno H, Baba M, Tanaka T, Nakamura S: Urokinase鈥抰ype plasminogen activator expression correlates with tumor angiogenesis and poor outcome in gastric cancer. / Cancer Sci 2003, 94:43鈥?9. CrossRef
14. Kita Y, Fukagawa T, Mimori K, Kosaka Y, Ishikawa K, Aikou T, Natsugoe S, Sasako M, Mori M: Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases. / Bri J Cancer 2008, 100:153鈥?59. CrossRef
15. Lee DH, Lee Y, Ryu J, Park SG, Cho S, Lee JJ, Choi C, Park BC: Identification of proteins differentially expressed in gastric cancer cells with high metastatic potential for invasion to lymph nodes. / Mol Cells 2011, 31:583鈥?71.
16. Zhang L, Zhao ZS, Ru GQ, Ma J: Correlative studies on uPA mRNA and uPAR mRNA expression with vascular endothelial growth factor, microvessel density, progression and survival time of patients with gastric cancer. / World J Gastroenterol 2006, 12:3970.
17. Ito H, Yonemura Y, Fujita H, Tsuchihara K, Kawamura T, Nojima N, Fujimura T, Nose H, Endo Y, Sasaki T: Prognostic relevance of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitors PAI-1 and PAI-2 in gastric cancer. / Virchows Arch 1996, 427:487鈥?96. CrossRef
18. Fujimura T, Kinami S, Ninomiya I, Kitagawa H, Fushida S, Nishimura G, Kayahara M, Shimizu K, Ohta T, Miwa K: Diagnostic laparoscopy, serum CA125, and peritoneal metastasis in gastric cancer. / Endoscopy 2002, 34:569鈥?74. CrossRef
19. Yonemura Y, Fujimura T, Ninomiya I, Kim BS, Bandou E, Sawa T, Kinoshita K, Endo Y, Sugiyama K, Sasaki T: Prediction of peritoneal micrometastasis by peritoneal lavaged cytology and reverse transcriptase-polymerase chain reaction for matrix metalloproteinase-7 mRNA. / Clin Cancer Res 2001, 7:1647鈥?653.
20. Fan B, Xiong B: Investigation of serum tumor markers in the diagnosis of gastric cancer. / Hepato-gastroenterol 2011, 58:239.
21. Ucar E, Semerci E, Ustun H, Yetim T, Huzmeli C, Gullu M: Prognostic value of preoperative CEA, CA 19鈥?, CA 72鈥?, and AFP levels in gastric cancer. / Adv Ther 2008, 25:1075鈥?084. CrossRef
22. Holst-Hansen C, Johannessen B, Hoyer-Hansen G, Romer J, Ellis V, Br眉nner N: Urokinase-type plasminogen activation in three human breast cancer cell lines correlates with their in vitro invasiveness. / Clin Exp Metastasis 1996, 14:297鈥?07.
23. Huang S, New L, Pan Z, Han J, Nemerow GR: Urokinase plasminogen activator/urokinase-specific surface receptor expression and matrix invasion by breast cancer cells requires constitutive p38伪 mitogen-activated protein kinase activity. / J Biol Chem 2000, 275:12266鈥?2272. CrossRef
24. Hsiao JK, Law B, Weissleder R, Tung CH: In-vivo imaging of tumor associated urokinase-type plasminogen activator activity. / J Biomed Optics 2006, 11:034013. CrossRef
25. Duffy M, Duggan C: The urokinase plasminogen activator system: a rich source of tumour markers for the individualised management of patients with cancer. / Clin Biochem 2004, 37:541鈥?48. CrossRef
26. Rosenthal EL, Johnson TM, Allen ED, Apel IJ, Punturieri A, Weiss SJ: Role of the plasminogen activator and matrix metalloproteinase systems in epidermal growth factor-and scatter factor-stimulated invasion of carcinoma cells. / Cancer Res 1998, 58:5221.
27. Heiss MM, Simon EH, Beyer BCM, Gruetzner KU, Tarabichi A, Babic R, Schildberg FW, Allgayer H: Minimal residual disease in gastric cancer: evidence of an independent prognostic relevance of urokinase receptor expression by disseminated tumor cells in the bone marrow. / J Clin Oncol 2002, 20:2005鈥?016. CrossRef
28. Okusa Y, Ichikura T, Mochizuki H: Prognostic impact of stromal cell鈥抎erived urokinase-type plasminogen activator in gastric carcinoma. / Cancer 1999, 85:1033鈥?038. CrossRef
29. Bogenrieder T, Herlyn M: Axis of evil: molecular mechanisms of cancer metastasis. / Oncogene 2003, 22:6524鈥?536. CrossRef
30. Saldanha RG, Molloy MP, Bdeir K, Cines DB, Song X, Uitto PM, Weinreb PH, Violette SM, Baker MS: Proteomic identification of lynchpin urokinase plasminogen activator receptor protein interactions associated with epithelial cancer malignancy. / J Proteome Res 2007, 6:1016鈥?028. CrossRef
- 作者单位：Youcheng Ding (1)
Hui Zhang (1)
Mingan Zhong (1)
Zhuqing Zhou (1)
Zhixiang Zhuang (1)
Hua Yin (1)
Xujing Wang (1)
Zhenggang Zhu (2)
1. Department of General Surgery, East Hospital, TongJi University School of Medicine, 150 Jimo Road, Shanghai, 200120, China
2. Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Road II, Shanghai, 200025, China
- ISSN：2047-783X
文摘
Background It has been demonstrated that urokinase-type plasminogen activator (uPA) is involved in tumor cell metastasis by degrading the extracellular matrix. However, there is little direct evidence of clinical uPA system expression in peritoneal metastatic tissues of gastric cancer. The objective of this study was to investigate uPA system expression in peritoneal tissues of peritoneal and nonperitoneal metastasis patients, and to explore the diagnostic value of the uPA system. Methods Expressions of uPA, uPAR, and PAI-1 were measured by semi-quantitative RT-PCR and ELISA. uPA activity was detected using a uPA activity kit. Results There was no significant difference in uPA, uPAR, and PAI-1 expression in two types of peritoneal tissue in seven patients with peritoneal metastasis. However, uPA, uPAR, and PAI-1 expressions in peritoneal metastatic lesions were significantly higher than those in normal peritoneal tissues of 24 nonperitoneal metastasis patients (P <0.05). Moreover, no statistical discrepancy of uPA activity was observed in various different tissues. Conclusions The expression of the uPA system positively correlates with peritoneal metastasis of gastric cancer. This expression difference in peritoneal or nonperitoneal metastasis patients may provide a reference for diagnosis of peritoneal metastasis.
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