Screening and transcriptome analysis of water deficiency tolerant germplasms in peanut (Arachis hypogaea)

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• 作者：Yi Shen ; E. Zhiguo ; Xiaojun Zhang ; Yonghui Liu ; Zhide Chen
• 关键词：Transcriptome sequencing ; Water deficiency stress ; Peanut ; Arachis hypogaea
• 刊名：Acta Physiologiae Plantarum
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：37
• 期：5
• 全文大小：1,497 KB
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• 作者单位：Yi Shen (1) (2)
  E. Zhiguo (3)
  Xiaojun Zhang (4)
  Yonghui Liu (1)
  Zhide Chen (1)
  
  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China
  2. Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China
  3. China National Rice Research Institute, No. 359, Tiyuchang Road, Hangzhou, 310006, China
  4. College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, 266109, China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Drought is one of the main limiting factors for peanut yield worldwide. However, the underlying genetic and molecular mechanisms remain unclear. In this study, we identified several water deficiency tolerant peanut germplasms based on physiological properties in the seedling stage. One of the germplasms was further characterized by transcriptome sequencing of samples that had been untreated or treated with PEG 6000 at three different times. A total of 370,145 non-redundant transcripts and 141,289 unigenes were obtained, and differentially expressed transcripts were identified among samples. Based on functional annotation, transcripts involved in drought response pathways were selected for qPCR analysis. Similar patterns but different levels of gene expression were found between drought-tolerant and drought-sensitive germplasms. These findings provide useful insights into drought tolerance of peanut.