Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.)

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• 作者：Shulin Xue (1) (2)
  Feng Xu (1) (2)
  Guoqiang Li (1) (2)
  Yan Zhou (1) (2)
  Musen Lin (1) (2)
  Zhongxia Gao (1) (2)
  Xiuhong Su (1) (2)
  Xiaowu Xu (1) (2)
  Ge Jiang (1) (2)
  Shuang Zhang (1) (2)
  Haiyan Jia (1) (2)
  Zhongxin Kong (1) (2)
  Lixia Zhang (1) (2)
  Zhengqiang Ma (1) (2)
  
• 刊名：Theoretical and Applied Genetics
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：126
• 期：8
• 页码：1941-1949
• 全文大小：487KB
• 参考文献：1. Bassam BJ, Gaetano-Anollé G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80-3 CrossRef
  2. Chen ML, Luo J, Shao GN, Wei XJ, Tang SQ, Sheng ZH, Song J, Hu PS (2012) Fine mapping of a major QTL for flag leaf width in rice, / qFLW4, which might be caused by alternative splicing of / NAL1. Plant Cell Rep 31:863-72 CrossRef
  3. Coleman RK, Gill GS, Rebetzke GJ (2001) Identification of quantitative trait loci for traits conferring weed competitiveness in wheat ( / Triticum aestivum L.). Aust J Agric Res 52:1235-246 CrossRef
  4. Cui KH, Peng SB, Xing YZ, Yu SB, Xu CG, Zhang QF (2003) Molecular dissection of the genetic relationships of source, sink and transport tissue with yield traits in rice. Theor Appl Genet 106:649-58
  5. Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295-07 CrossRef
  6. Farooq M, Tagle AG, Santos RE, Ebron LA, Fujita D, Kobayashi N (2010) Quantitative trait loci mapping for leaf length and leaf width in rice cv. IR64 derived lines. J Integr Plant Biol 52:578-84 CrossRef
  7. Fujino K, Matsuda Y, Ozawa K, Nishimura T, Koshiba T, Fraaije MW, Sekiguchi H (2008) / NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics 279:499-07 CrossRef
  8. Gasperini D, Greenland A, Hedden P, Dreos R, Harwood W, Griffiths S (2012) Genetic and physiological analysis of / Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. J Exp Bot 63:4419-436 CrossRef
  9. Groos C, Robert N, Bervas E, Charmet G (2003) Genetic analysis of grain protein-content, grain yield and thousand-kernel weight in bread wheat. Theor Appl Genet 106:1032-040
  10. Hospital F (2001) Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs. Genetics 158:1363-379
  11. Jia HY, Wan HS, Yang SH, Zhang ZZ, Kong ZX, Xue SL, Zhang LX, Ma ZQ (2013) Genetic dissection of yield-related traits in a recombinant inbred line population created using a key breeding parent in China’s wheat breeding. Theor Appl Genet (Accepted)
  12. Kato K, Miura H, Sawada S (2000) Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor Appl Genet 101:1114-121 CrossRef
  13. Khaliq I, Irshad A, Ahsan M (2008) Awns and flag leaf contribution towards grain yield in spring wheat ( / Triticum aestivum L.). Cereal Res Commun 36:65-6 CrossRef
  14. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172-75
  15. Lin F, Xue SL, Zhang ZZ, Zhang CQ, Kong ZX, Yao GQ, Tian DG, Zhu HL, Li CJ, Cao Y, Wei JB, Luo QY, Ma ZQ (2006) Mapping QTL associated with resistance to / Fusarium head blight in the Nanda2419?×?Wangshuibai population. II: type I resistance. Theor Appl Genet 112:528-35 CrossRef
  16. Lincoln SE, Daly MJ, Lander ES (1992) Constructing genetic maps with MAPMAKER/EXP Version 3.0. Technical Report, 3rd edn. Whitehead Institute, Cambridge, MA
  17. Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas 25:317-21
  18. Ma ZQ, Sorrells ME (1995) Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci 35:1137-143 CrossRef
  19. Ma ZQ, R?der MS, Sorrells ME (1996) Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome 39:123-30 CrossRef
  20. Ma ZQ, Xue SL, Lin F, Yang SH, Li GQ, Tang MZ, Kong ZX, Cao Y, Zhao DM, Jia HY, Zhang ZZ, Zhang LX (2008) Mapping and validation of scab resistance QTLs in the Nanda2419?×?Wangshuibai population. Cereal Res Commun 36(Suppl B):245-51 CrossRef
  21. Mei HW, Luo LJ, Ying CS, Wang YP, Yu XQ, Guo LB, Paterson AH, Li ZK (2003) Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations. Theor Appl Genet 107:89-01
  22. Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution-genes encode mutant gibberellin response modulators. Nature 400:256-61 CrossRef
  23. Qi J, Qian Q, Bu Q, Li S, Chen Q, Sun J, Liang W, Zhou Y, Chu C, Li X, Ren F, Palme K, Zhao B, Chen J, Chen M, Li C (2008) Mutation of the rice / narrow / leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol 147:1947-959 CrossRef
  24. Qiu XJ, Gong R, Tan YB, Yu SB (2012) Mapping and characterization of the major quantitative trait locus / qSS7 associated with increased length and decreased width of rice seeds. Theor Appl Genet 125:1717-726 CrossRef
  25. Quarrie SA, Steed A, Calestani C, Semikhidskii A, Lebreton C, Chinoy C, Steele N, Pljevljakusic D, Waterman E, Weyen J, Schondelmaier J, Habash DZ, Farmer P, Saker L, Clarkson DT, Abugalieva A, Yessimbekova M, Turuspekov Y, Abugalieva S, Tuberosa R, Sanguineti MC, Hollington PA, Aragues R, Royo A, Dodig D (2005) A high-density genetic map of hexaploid wheat ( / Triticum aestivum L.) from the cross Chinese Spring?×?SQ1 and its use to compare QTLs for grain yield across a range of environments. Theor Appl Genet 110:865-80 CrossRef
  26. R?der MS, Korzun V, Wendehake K, Plaschke J, Tixer MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007-023
  27. ?afá? J, ?imková H, Kubaláková M, ?íhalíková J, Suchánková P, Barto? J, Dole?el J (2010) Development of chromosome-specific BAC resources for genomics of bread wheat. Cytogenet Genome Res 129:211-23 CrossRef
  28. Sharma SN, Sain RS, Sharma PK (2003) The genetic control of flag leaf length in normal and late sown durum wheat. J Agr Sci 141:323-31 CrossRef
  29. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat ( / Triticum aestivum L.). Theor Appl Genet 109:1105-114 CrossRef
  30. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550-60 CrossRef
  31. Wang P, Zhou GL, Yu HH, Yu SB (2011) Fine mapping a major QTL for flag leaf size and yield-related traits in rice. Theor Appl Genet 123:1319-330 CrossRef
  32. Wang S, Wu K, Yuan Q, Liu X, Liu Z, Lin X, Zeng R, Zhu H, Dong G, Qian Q, Zhang G, Fu X (2012) Control of grain size, shape and quality by / OsSPL16 in rice. Nat Genet 44:950-54 CrossRef
  33. Xing YZ, Tang WJ, Xue WY, Xu CG, Zhang QF (2008) Fine mapping of a major quantitative trait loci, / qSSP7, controlling the number of spikelets per panicle as a single Mendelian factor in rice. Theor Appl Genet 116:789-96 CrossRef
  34. Xu HY, Zhao JS (1995) Canopy photosynthesis capacity and the contribution from different organs in high-yielding winter wheat. Acta Agro Sin 21:204-09 (in Chinese with English abstract)
  35. Xue DW, Chen MC, Zhou MX, Chen S, Mao Y, Zhang GP (2008a) QTL analysis of flag leaf in barley ( / Hordeum vulgare L.) for morphological traits and chlorophyll content. J Zhejiang Univ Sci B 9:938-43 CrossRef
  36. Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhao DM, Zhu HL, Xu HB, Wu JZ, Tian DG, Zhang CQ, Ma ZQ (2008b) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181-89 CrossRef
  37. Xue SL, Li GQ, Jia HY, Lin F, Cao Y, Xu F, Tang MZ, Wang Y, Wu XY, Zhang ZZ, Zhang LX, Kong ZX, Ma ZQ (2010) Marker-assisted development and evaluation of near-isogenic lines for scab resistance QTLs of wheat. Mol Breeding 25:397-05 CrossRef
  38. Xue SL, Xu F, Tang MZ, Zhou Y, Li GQ, An X, Lin F, Xu HB, Jia HY, Zhang LX, Kong ZX, Ma ZQ (2011) Precise mapping / Fhb5, a major QTL conditioning resistance to / Fusarium infection in bread wheat ( / Triticum aestivum L.). Theor Appl Genet 123:1055-063 CrossRef
  39. Yang DL, Jing RL, Chang XP, Li W (2007) Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat ( / Triticum aestivum). J Integr Plant Biol 49:646-54 CrossRef
  40. Yue B, Xue WY, Luo LJ, Xing YZ (2006) QTL analysis for flag leaf characteristics and their relationships with yield and yield traits in rice. Acta Genet Sin 33:824-32 CrossRef
  41. Zhang LY, Liu DC, Guo XL, Yang WL, Sun JZ, Wang DW, Zhang AM (2010) Genomic distribution of quantitative trait loci for yield and yield-related traits in common wheat. J Integr Plant Biol 52:996-007 CrossRef
  42. Zhou L, Chen L, Jiang L, Zhang W, Liu L, Liu X, Zhao Z, Liu S, Zhang L, Wang J, Wan J (2009) Fine mapping of the grain chalkiness QTL / qPGWC- / 7 in rice ( / Oryza sativa L.). Theor Appl Genet 118:581-90 CrossRef
  
• 作者单位：Shulin Xue (1) (2)
  Feng Xu (1) (2)
  Guoqiang Li (1) (2)
  Yan Zhou (1) (2)
  Musen Lin (1) (2)
  Zhongxia Gao (1) (2)
  Xiuhong Su (1) (2)
  Xiaowu Xu (1) (2)
  Ge Jiang (1) (2)
  Shuang Zhang (1) (2)
  Haiyan Jia (1) (2)
  Zhongxin Kong (1) (2)
  Lixia Zhang (1) (2)
  Zhengqiang Ma (1) (2)
  
  1. The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China
  2. College of Agricultural Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China
  
• ISSN：1432-2242

文摘

Introduction Flag leaf width (FLW) is directly related to photosynthetic capacity and yield potential in wheat. In a previous study, Qflw.nau-5A controlling FLW was detected on chromosome 5A in the interval possessing Fhb5 for type I Fusarium head blight (FHB) resistance using a recombinant inbred line population derived from Nanda2419 × Wangshuibai. Materials and methods Qflw.nau-5A near-isogenic line (NIL) with the background of Mianyang 99-323 and PH691 was developed and evaluated. FLW inheritance was investigated using two F2 populations developed from crossing the Qflw.nau-5A NILs with their recurrent parents. One hundred ten and 28 recombinants, which included 10 and 5 types of recombinants, were identified from 2816 F2 plants with Mianyang 99-323 background and 1277 F2 plants with PH691 background, respectively, and phenotyped in field trials for FLW and type I FHB resistance. Deletion bin mapping was applied to physically map Qflw.nau-5A. Results and conclusions The introduction of Wangshuibai Qflw.nau-5A allele reduced the FLW up to 3 mm. In the F2 populations, Qflw.nau-5A was inherited like a semi-dominant gene, and was therefore designated as TaFLW1. The FLW of the recombinant lines displayed a distinct two-peak distribution. Recombinants with wider leaves commonly have Mianyang 99-323 or PH691 chromatin in the 0.2 cM Xwmc492-Xwmc752 interval that resided in the 5AL12-0.35-.57 deletion bin, and recombinants with narrow leaves were Wangshuibai genotype in this interval. Phenotypic recombination between FLW and type I FHB resistance was identified, implying TaFLW1 was in close linkage with Fhb5. These results should aid wheat breeders to break the linkage drag through marker-assisted selection and assist in the map-based cloning of TaFLW1.