Saliva as a sampling source for the detection of leukemic fusion transcripts

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• 作者：Dongmei Chen (1) (2)
  Najie Song (1) (2)
  Runfang Ni (1) (2)
  Jiangning Zhao (3)
  Jiasheng Hu (3)
  Quanyi Lu (3)
  Qingge Li (1) (2)
  
  1. State Key Laboratory of Cellular Stress Biology ; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics ; Engineering Research Centre of Molecular Diagnostics ; Ministry of Education ; School of Life Sciences ; Xiamen University ; Xiamen ; Fujian ; 361102 ; China
  2. Shenzhen Research Institute of Xiamen University ; Shenzhen ; Guangdong ; 518057 ; China
  3. Zhongshan Hospital of Xiamen ; Xiamen University ; Xiamen ; Fujian ; 361004 ; China
  
• 关键词：Leukemia ; Leukemic fusion transcript ; Saliva ; Molecular diagnosis ; Minimal residual disease
• 刊名：Journal of Translational Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：12
• 期：1
• 全文大小：1,417 KB
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• 刊物主题：Biomedicine general; Medicine/Public Health, general;
• 出版者：BioMed Central
• ISSN：1479-5876

文摘

Background Saliva has long been used as a sampling source for clinical diagnosis of oral disease such as oral squamous cell carcinoma, or therapeutic drug monitoring. The aims of this study was to ascertain if saliva RNA could be stored at room temperature and to study if saliva could be a convenient source for fusion transcripts in leukemic patients. Methods This is a cross-sectional diagnostic study. We first developed a Saliva RNA tube for stable storage of whole saliva RNA at room temperature. Then we detected the leukemic fusions in the whole saliva from seven leukemic patients and twenty healthy volunteers, and compared with the results obtained from the bone marrow of the patients. Results Human gene transcripts could be reproducibly detected in the whole saliva for at least four weeks when stored in the developed composition at room temperature. Concordant results of the fusion transcripts were obtained between the saliva and the bone marrow in the seven leukemic patients and no fusions were detected in the healthy controls. Conclusions The results support our hypothesis that human whole saliva could be a reliable and convenient sampling source for the detection of leukemic fusions.