Poly(lactic-co-glycolic) acid nanoparticles uptake by Vitis vinifera and grapevine-pathogenic fungi

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• 作者：Alessio Valletta (1)
  Laura Chronopoulou (2)
  Cleofe Palocci (2)
  Barbara Baldan (3)
  Livia Donati (1)
  Gabriella Pasqua (1)
  
• 关键词：Polymeric nanoparticles ; Plant cell culture ; Vitis vinifera ; Transmission electron microscopy
• 刊名：Journal of Nanoparticle Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：16
• 期：12
• 全文大小：2,360 KB
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• 作者单位：Alessio Valletta (1)
  Laura Chronopoulou (2)
  Cleofe Palocci (2)
  Barbara Baldan (3)
  Livia Donati (1)
  Gabriella Pasqua (1)
  
  1. Department of Environmental Biology, “Sapienza-University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy
  2. Department of Chemistry, “Sapienza-University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy
  3. Department of Biology, University of Padua, Via Ugo Bassi 58/B, 35131, Padua, Italy
  
• ISSN：1572-896X

文摘

Poly(lactic-co-glycolic) acid (PLGA)-based NPs are currently considered among the most promising drug carriers, nevertheless their use in plants has never been investigated. In this work, for the first time, we demonstrated the ability of PLGA NPs to cross the plant cell wall and membrane of Vitis vinifera cell cultures and grapevine-pathogenic fungi. By means of fluorescence microscopy, we established that PLGA NPs can enter in grapevine leaf tissues through stomata openings and that they can be absorbed by the roots and transported to the shoot through vascular tissues. TEM analysis on cultured cells showed that NPs?≤?0?nm could enter cells, while bigger ones remained attached to the cell wall. Viability tests demonstrated that PLGA NPs were not cytotoxic for V. vinifera-cultured cells. The cellular uptake of PLGA NPs by some important grapevine-pathogenic fungi has also been observed, thus suggesting that PLGA NPs could be used to deliver antifungal compounds within fungal cells. Overall the results reported suggest that such NPs may play a key role in future developments of agrobiotechnologies, as it is currently happening in biomedicine.