Time-lapse observations to analyze the effects of assisted hatching

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• 作者：Maki Goto ; Akira Iwase ; Naomi Furusawa…
• 关键词：Assisted hatching ; Blastocyst ; Contraction ; Hatching ; Time ; lapse observation
• 刊名：Reproductive Medicine and Biology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：13
• 期：4
• 页码：217-221
• 全文大小：220 KB
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• 作者单位：Maki Goto (1)
  Akira Iwase (1) (2)
  Naomi Furusawa (3)
  Harumi Kobayashi (3)
  Nao Kato (1)
  Ai Saito (1)
  Masahiko Mori (1)
  Satoko Osuka (1)
  Mika Kondo (1)
  Tomoko Nakamura (1)
  Tatsuo Nakahara (1)
  Hiroyuki Matsumoto (3)
  Fumitaka Kikkawa (1)
  
  1. Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan
  2. Department of Maternal and Perinatal Medicine, Nagoya University Hospital, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan
  3. Department of Medical Technique, Nagoya University Hospital, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan
  
• ISSN：1447-0578

文摘

Purpose Assisted hatching (AH) is an artificial disruption of the zona pellucida with the aim of facilitating embryo implantation. We used time-lapse observations of mouse embryos to examine the effect of AH in mouse blastocysts. Methods AH techniques were performed with acid Tyrode’s solution. We compared the rates of blastocyst formation and blastocyst attachment to Ishikawa cells between the control (n?=?28) and the AH group (n?=?24). To analyze the effects of AH, 8-cell mice embryos were cultured under time-lapse observations (every 15?min). The time required for hatching, the hatching rates, the frequency of contraction, and the contraction rates in the blastocysts were analyzed. Results There were no significant differences between the two groups in hatching rate or attachment rate. The times required for hatching were 286?±?22?min in the AH group and 990?±?437?min in the control group (P?=?0.018). The contraction frequencies in blastocysts were 3.5?±?0.7 times in the AH group and 7.5?±?2.5 times in the control group (P?=?0.020). Conclusions From the time-lapse observations we found that the time required for hatching and the frequency of contraction in blastocysts were both reduced by AH, although blastocyst formation and attachment were not affected.