Imaging oxygen in neural cell and tissue models by means of anionic cell-permeable phosphorescent nanoparticles

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• 作者：Ruslan I. Dmitriev (1)
  Sergey M. Borisov (2)
  Alina V. Kondrashina (1)
  Janelle M. P. Pakan (3)
  Ujval Anilkumar (4)
  Jochen H. M. Prehn (4)
  Alexander V. Zhdanov (1)
  Kieran W. McDermott (3)
  Ingo Klimant (2)
  Dmitri B. Papkovsky (1)
  
• 关键词：Biomaterials ; Cell and tissue oxygen ; Intracellular oxygen probe ; Multiplexed detection ; Nanosensors ; Phosphorescence quenching microscopy ; PLIM
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：72
• 期：2
• 页码：367-381
• 全文大小：4,625 KB
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• 作者单位：Ruslan I. Dmitriev (1)
  Sergey M. Borisov (2)
  Alina V. Kondrashina (1)
  Janelle M. P. Pakan (3)
  Ujval Anilkumar (4)
  Jochen H. M. Prehn (4)
  Alexander V. Zhdanov (1)
  Kieran W. McDermott (3)
  Ingo Klimant (2)
  Dmitri B. Papkovsky (1)
  
  1. School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland
  2. Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9, 8010, Graz, Austria
  3. Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland
  4. Department of Physiology and Medical Physics, Centre for the Study of Neurological Disorders, Royal College of Surgeons in Ireland, Dublin, Ireland
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

Cell-permeable phosphorescent probes enable the study of cell and tissue oxygenation, bioenergetics, metabolism, and pathological states such as stroke and hypoxia. A number of such probes have been described in recent years, the majority consisting of cationic small molecule and nanoparticle structures. While these probes continue to advance, adequate staining for the study of certain cell types using live imaging techniques remains elusive; this is particularly true for neural cells. Here we introduce novel probes for the analysis of neural cells and tissues: negatively charged poly(methyl methacrylate-co-methacrylic acid)-based nanoparticles impregnated with a phosphorescent Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP) dye (this form is referred to as PA1), and with an additional reference/antennae dye poly(9,9-diheptylfluorene-alt-9,9-di-p-tolyl-9H-fluorene) (this form is referred to as PA2). PA1 and PA2 are internalised by endocytosis, result in efficient staining in primary neurons, astrocytes, and PC12 cells and multi-cellular aggregates, and allow for the monitoring of local O2 levels on a time-resolved fluorescence plate reader and PLIM microscope. PA2 also efficiently stains rat brain slices and permits detailed O2 imaging experiments using both one and two-photon intensity-based modes and PLIM modes. Multiplexed analysis of embryonic rat brain slices reveals age-dependent staining patterns for PA2 and a highly heterogeneous distribution of O2 in tissues, which we relate to the localisation of specific progenitor cell populations. Overall, these anionic probes are useful for sensing O2 levels in various cells and tissues, particularly in neural cells, and facilitate high-resolution imaging of O2 in 3D tissue models.