Screening and identification of organics-degrading bacteria from the sediment of sea cucumber Apostichopus japonicus ponds

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• 作者：Dongsheng Zhang ; Hua Li ; Yang Liu ; Guo Qiao ; Shuang Chi…
• 关键词：Apostichopus japonicus ; Pond sediment ; Screening ; Probiotics ; Bacillus ; Pseudomonas spp.
• 刊名：Aquaculture International
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：24
• 期：1
• 页码：373-384
• 全文大小：490 KB
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• 作者单位：Dongsheng Zhang (1)
  Hua Li (1)
  Yang Liu (1)
  Guo Qiao (2)
  Shuang Chi (1)
  Jian Song (1)
  
  1. Key Laboratory of Mariculture and Stock Enhancement North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, Liaoning, China
  2. Department of Ocean Technology, College of Chemistry and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  
• 出版者：Springer Netherlands
• ISSN：1573-143X

文摘

The organics-degrading bacteria were isolated from sea cucumber (Apostichopus japonicus) outdoor rearing ponds sediment. The bacterial strains were selected according to the isolation growth, pathogenicity, organic degradation rate, and effects on A. japonicus growth and water quality. The screened strains were identified on physiological, biochemical, and genetic characteristics. Among 68 strains isolated, two strains (L15 and E4) were identified as potential organics-degrading probiotics. The degradation rates on sediment chemical oxygen demand (COD) were 46.95 ± 1.58 % for the L15 strain and 44.31 ± 1.44 % for the E4 strain. The L15 strain enhanced the specific growth rate (SGR) of A. japonicus by 1.97 ± 0.49 %, and the E4 strain enhanced SGR by 2.24 ± 0.44 % compared with the SGR of 0.55 ± 0.41 % in the control group without probiotics. The COD level increased by −0.9 ± 1.42 % of L15 and 47.9 ± 0.06 % of E4 inoculations after 7 days, while the COD increased by 140.6 ± 4.22 % in the control. Based on the analyses of biochemical characteristics and 16S rRNA gene sequences, the L15 and E4 strains were identified as Bacillus spp. and Pseudomonas spp., respectively. Keywords Apostichopus japonicus Pond sediment Screening Probiotics Bacillus Pseudomonas spp.