刊物主题:Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
出版者:Springer Vienna
ISSN:1436-5073
卷排序:184
文摘
Shrimp allergy is a common cause of allergenic diseases. The authors describe an aptamer-based assay for tropomyosin, one of the major allergens of shrimp. After six rounds of SELEX based on nitrocellulose membrane, several aptamers with high affinity (Kd = 109.6 nM) were identified that can selectively recognize tropomyosin but not other proteins over a large range of concentrations. By using a fluorescent and graphene oxide based platform, a tropomyosin assay was worked out that has a 4.2 nM detection limit and work in the 0.5 to 50 μg·mL−1 concentration range. The aptamers selected hold a great potential for tropomyosin detection in practical biosensor assays.
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