DNA aptamer for use in a fluorescent assay for the shrimp allergen tropomyosin

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• 作者：Youxiong Zhang ; Qingping Wu ; Xianhu Wei ; Jumei Zhang ; Shuping Mo
• 关键词：Biosensor ; Allergen detection ; Graphene oxide ; Tetramethylbenzidine ; Horse radish peroxidase ; Binding assay
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：184
• 期：2
• 页码：633-639
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

Shrimp allergy is a common cause of allergenic diseases. The authors describe an aptamer-based assay for tropomyosin, one of the major allergens of shrimp. After six rounds of SELEX based on nitrocellulose membrane, several aptamers with high affinity (K_d = 109.6 nM) were identified that can selectively recognize tropomyosin but not other proteins over a large range of concentrations. By using a fluorescent and graphene oxide based platform, a tropomyosin assay was worked out that has a 4.2 nM detection limit and work in the 0.5 to 50 μg·mL−1 concentration range. The aptamers selected hold a great potential for tropomyosin detection in practical biosensor assays.