Overexpression of a Miscanthus lutarioriparius NAC gene MlNAC5 confers enhanced drought and cold tolerance in Arabidopsis

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• 作者：Xuanwen Yang ; Xiaoyu Wang ; Lu Ji ; Zili Yi ; Chunxiang Fu…
• 关键词：Miscanthus lutarioriparius ; Abiotic stress ; Abscisic acid ; Bioenergy crop ; NAC transcription factor
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：34
• 期：6
• 页码：943-958
• 全文大小：3,278 KB
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• 作者单位：Xuanwen Yang (1) (2)
  Xiaoyu Wang (2)
  Lu Ji (3)
  Zili Yi (4)
  Chunxiang Fu (2)
  Jingcheng Ran (1)
  Ruibo Hu (2)
  Gongke Zhou (2)
  
  1. College of Life Sciences, Guizhou Normal University, Guiyang, 550001, Guizhou, People鈥檚 Republic of China
  2. CAS Key Laboratory of Biofuels and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People鈥檚 Republic of China
  3. Laboratory of Basic Biology, Hunan First Normal University, Changsha, 410205, Hunan, People鈥檚 Republic of China
  4. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message MLNAC5 functions as a stress-responsive NAC transcription factor gene and enhances drought and cold stress tolerance in transgenic Arabidopsis via the ABA-dependent signaling pathway. Abstract NAC transcription factors (TFs) play crucial roles in plant responses to abiotic stress. Miscanthus lutarioriparius is one of Miscanthus species native to East Asia. It has attracted much attention as a bioenergy crop because of its superior biomass productivity as well as wide adaptability to different environments. However, the functions of stress-related NAC TFs remain to be elucidated in M. lutarioriparius. In this study, a detailed functional characterization of MlNAC5 was carried out. MlNAC5 was a member of ATAF subfamily and it showed the highest sequence identity to ATAF1. Subcellular localization of MlNAC5-YFP fusion protein in tobacco leaves indicated that MlNAC5 is a nuclear protein. Transactivation assay in yeast cells demonstrated that MlNAC5 functions as a transcription activator and its activation domain is located in the C-terminus. Overexpression of MlNAC5 in Arabidopsis had impacts on plant development including dwarfism, leaf senescence, leaf morphology, and late flowering under normal growth conditions. Furthermore, MlNAC5 overexpression lines in Arabidopsis exhibited hypersensitivity to abscisic acid (ABA) and NaCl. Moreover, overexpression of MlNAC5 in Arabidopsis significantly enhanced drought and cold tolerance by transcriptionally regulating some stress-responsive marker genes. Collectively, our results indicated that MlNAC5 functions as an important regulator during the process of plant development and responses to salinity, drought and cold stresses.