Bioaugmentation with Novel Microbial Formula vs. Natural Attenuation of a Long-Term Mixed Contaminated Soil鈥擳reatability Studies in Solid- and Slurry-Phase Microcosms

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• 作者：Saranya Kuppusamy ; Palanisami Thavamani…
• 关键词：Manufactured gas plant (MGP) ; Soil bioremediation ; Polycyclic aromatic hydrocarbons (PAHs) ; Heavy metals ; Natural attenuation ; Bacterial consortium ; Bioaugmentation
• 刊名：Water, Air, and Soil Pollution
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：227
• 期：1
• 全文大小：1,687 KB
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• 作者单位：Saranya Kuppusamy (1) (2)
  Palanisami Thavamani (2) (3)
  Mallavarapu Megharaj (2) (3)
  Ravi Naidu (2) (3)
  
  1. Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA, 5095, Australia
  2. Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE), PO Box 486, Salisbury South, SA, 5106, Australia
  3. Global Centre for Environmental Remediation (GCER), Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Terrestrial Pollution
  Hydrogeology
  
• 出版者：Springer Netherlands
• ISSN：1573-2932

文摘

Treatability studies in real contaminated soils are essential to predict the feasibility of microbial consortium augmentation for field-scale bioremediation of contaminated sites. In this study, the biodegradation of a mixture of seven PAHs in a manufactured gas plant (MGP) soil contaminated with 3967 mg kg鈭? of total PAHs using novel acid-, metal-tolerant, N-fixing, P-solubilizing, and biosurfactant-producing LMW and HMW PAH-degrading bacterial combinations as inoculums was compared in slurry- and solid-phase microcosms over natural attenuation. Bioaugmentation of 5 % of bacterial consortia A and N in slurry- and solid-phase systems enhanced 4.6鈥?.7 and 9.3鈥?0.7 % of total PAH degradation, respectively, over natural attenuation. Occurrence of 62.7鈥?8 % of PAH biodegradation during natural attenuation in soil and slurry illustrated the accelerated rate of intrinsic metabolic activity of the autochthonous microbial community in the selected MGP soil. Monitoring of the total microbial activity and population of PAH degraders revealed that the observed biodegradation trend in MGP soil resulted from microbial mineralization. In the slurry, higher biodegradation rate constant (k) and lower half-life values (t _1/2) was observed during bioaugmentation with consortium N, highlighting the use of bioaugmentation in bioslurries/bioreactor to achieve rapid and efficient bioremediation compared to that of a static solid system. In general, natural attenuation was on par with bioaugmentation. Hence, depending on the type of soil, natural attenuation might outweigh bioaugmentation and a careful investigation using laboratory treatability studies are highly recommended before the upscale of a developed bioremediation strategy to field level. Keywords Manufactured gas plant (MGP) Soil bioremediation Polycyclic aromatic hydrocarbons (PAHs) Heavy metals Natural attenuation Bacterial consortium Bioaugmentation