Beauveria bassiana as an endophyte: a critical review on associated methodology and biocontrol potential

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• 作者：Aimee C. McKinnon ; Susanna Saari ; Maria E. Moran-Diez ; Nicolai V. Meyling…
• 关键词：Entomopathogenic fungi ; Inoculation ; Isolation ; Herbivore ; Pest ; Cultivation ; dependent ; Cultivation ; independent detection ; Surface disinfection ; Insect
• 刊名：BioControl
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：62
• 期：1
• 页码：1-17
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Entomology; Plant Pathology; Agriculture; Animal Ecology; Animal Biochemistry; Behavioral Sciences;
• 出版者：Springer Netherlands
• ISSN：1573-8248
• 卷排序：62

文摘

In the last decade there has been increased focus on the potential of endophytic Beauveria bassiana for the biocontrol of insect herbivores. Generally, detection of endophytes is acknowledged to be problematic and recovery method-dependent. Herein, we critically analyse the methodology reported for the detection of B. bassiana as endophytes following experimental inoculation. In light of the methodology, we further review the effects of endophytic B. bassiana on insect herbivores. Our review indicated the need for stringent protocols for surface sterilisation including thorough experimental controls. For molecular detection protocols by PCR, residual DNA from surface inocula must also be considered. The biocontrol potential of B. bassiana endophytes appears promising although both negative and neutral effects on insect herbivores were reported and there remains ambiguity with respect to the location and mode of action of the fungus in planta. We recommend that future studies adopt multiple techniques, including culture dependent and independent techniques for endophyte detection and elucidate the mechanisms involved against insect herbivores.