Expression of calpain-calpastatin system (CCS) member proteins in human lymphocytes of young and elderly individuals; pilot baseline data for the CALPACENT project

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• 作者：Anna Mikosik (5)
  Jerzy Foerster (4)
  Aleksandra Jasiulewicz (5)
  Joanna Fr?ckowiak (5)
  Giuseppina Colonna-Romano (5)
  Matteo Bulati (5)
  Silvio Buffa (5)
  Adriana Martorana (5)
  Calogero Caruso (5)
  Ewa Bryl (5)
  Jacek M Witkowski (5)
  
• 关键词：Ageing ; μ ; Calpain ; m ; calpain ; Calpastatin ; Human ; Lymphocytes ; Quantitative flow cytometry
• 刊名：Immunity & Ageing
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：10
• 期：1
• 全文大小：297KB
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• 作者单位：Anna Mikosik (5)
  Jerzy Foerster (4)
  Aleksandra Jasiulewicz (5)
  Joanna Fr?ckowiak (5)
  Giuseppina Colonna-Romano (5)
  Matteo Bulati (5)
  Silvio Buffa (5)
  Adriana Martorana (5)
  Calogero Caruso (5)
  Ewa Bryl (5)
  Jacek M Witkowski (5)
  
  5. Department of Biopathology and Medical and Forensic Biotechnologies (DIBIMEF), University of Palermo, Palermo, Italy
  4. Department of Social and Clinical Gerontology, Medical University of Gdańsk, Gdańsk 7, Poland
  

文摘

Background Ubiquitous system of regulatory, calcium-dependent, cytoplasmic proteases -calpains -and their endogenous inhibitor -calpastatin -is implicated in the proteolytic regulation of activation, proliferation, and apoptosis of many cell types. However, it has not been thoroughly studied in resting and activated human lymphocytes yet, especially in relation to the subjects-ageing process. The CALPACENT project is an international (Polish-Italian) project aiming at verifying the hypothesis of the role of calpains in the function of peripheral blood immune cells of Polish (Pomeranian) and Italian (Sicilian) centenarians, apparently relatively preserved in comparison to the general elderly population. In this preliminary report we aimed at establishing and comparing the baseline levels of expression of μ- and m-calpain and calpastatin in various, phenotypically defined, populations of human peripheral blood lymphocytes for healthy elderly Sicilians and Poles, as compared to these values observed in young cohort. Results We have found significant differences in the expression of both μ- and m-calpain as well as calpastatin between various populations of peripheral blood lymphocytes (CD4+, CD8+ and CD19+), both between the age groups compared and within them. Interestingly, significantly higher amounts of μ- and m-calpains but not of calpastatin could be demonstrated in the CD4+CD28- and CD8+CD28- lymphocytes of old subjects (but not in the cells of young individuals), as compared to their CD28+ counterparts. Finally, decreased expression of both calpains in the elderly T cells is not related to the accumulation of effector/memory (CD45RO+) cells in the latter, as the expression of both calpains does not differ significantly between the na?ve and memory T cells, while is significantly lower for elderly lymphocytes if both populations are taken separately. Conclusions Observed differences in the amounts of CCS member proteins between various populations of lymphocytes of young and elderly subjects may participate in the impaired proliferative activity of these cells in the elderly.