Characterization of an acsD disruption mutant provides additional evidence for the hierarchical cell-directed self-assembly of cellulose in Gluconacetobacter xylinus

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• 作者：Kalpa Mehta ; Sarah Pfeffer ; R. Malcolm Brown Jr.
• 关键词：Cellulose ; Microbial cellulose ; Hierarchical assembly of cellulose ; Gluconacetobacter xylinus ; Acetobacter xylinum ; acsD ; acsD mutant ; Linear terminal complex ; Terminal complex
• 刊名：Cellulose
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：22
• 期：1
• 页码：119-137
• 全文大小：1,634 KB
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• 作者单位：Kalpa Mehta (1)
  Sarah Pfeffer (1)
  R. Malcolm Brown Jr. (1)
  
  1. Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, 100 West 24th Street, Austin, TX, 78712, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Bioorganic Chemistry
  Physical Chemistry
  Organic Chemistry
  Polymer Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-882X

文摘

The acsD gene is involved in cellulose biosynthesis among the Acetobacter species. In the current study, we created an acsD disruption mutant in the acsABCD cellulose synthase operon of Gluconacetobacter xylinus and characterized the resulting cellulose to aid in providing insight into the function of the acsD gene. Both the wild type G. xylinus AY201 (derivative of Gluconacetobacter hansenii ATCC 23769) and the acsD disruption mutant produced crystalline cellulose I microfibrils. The cellulose produced by both appeared to be synthesized from an aggregate of pores known as a linear terminal complex; however the total cellulose synthesized was 10?% that of the wild type G. xylinus AY201. TEM observations of the acsD disruption mutant confirmed that microfibrils and bundles of microfibrils were similar in size to the G. xylinus AY201 wild type; however, the final ribbon dimensions were narrower (53.4?±?13.1?nm wt, vs. 28.2?±?8.2?nm). Additional TEM observations of the mutant cells incubated at 4?°C revealed an abnormal linear terminal complex orientation whereby the resulting band material could be observed in a transverse orientation as well as longitudinally to the long axis of the cell. Taken together, these data strongly suggest that acsD aids in the proper orientation of the linear terminal complexes along the longitudinal axis of the cell indicating the AcsD protein is involved in the final level of the hierarchical assembly of cellulose resulting in highly efficient cellulose synthesis.