Protective effect of epigallocatechin gallate, a major constituent of green tea, against renal ischemia–reperfusion injury in rats

详细信息    查看全文

• 作者：Jun Lv ; Min Feng ; LiLi Zhang ; Xia Wan ; Yu Chun Zeng…
• 关键词：EGCG ; Inflammation ; Apoptosis ; Renal ischemia–reperfusion injury
• 刊名：International Urology and Nephrology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：47
• 期：8
• 页码：1429-1435
• 全文大小：3,440 KB
• 参考文献：1.Wang Y, Seto SW, Golledge J (2013) Therapeutic effects of renal denervation on renal failure. Curr Neurovasc Res 10:172-84PubMed View Article
  2.Kher A, Meldrum KK, Wang M, Tsai BM, Pitcher JM, Meldrum DR (2005) Cellular and molecular mechanisms of sex differences in renal ischemia-reperfusion injury. Cardiovasc Res 67:594-03PubMed View Article
  3.Daemen MA, de Vries B, Buurman WA (2002) Apoptosis?and?inflammation?in?renal?reperfusion injury. Transplantation 2002(15):1693-700View Article
  4.Rogers NM, Stephenson MD, Kitching AR, Horowitz JD, Coates PT (2012) Amelioration of?renal?ischaemia-reperfusion injury?by liposomal delivery of curcumin to?renal tubular epithelial and antigen-presenting cells. Br J Pharmacol 166:194-09PubMed Central PubMed View Article
  5.Kao WP, Yang CY, Su TW, Wang YT, Lo YC, Lin SC (2015) The versatile roles of CARDs in regulating?apoptosis,?inflammation, and?NF-κB signaling. Apoptosis 20:174-95PubMed View Article
  6.Pae M, Wu D (2013) Immunomodulating effects of epigallocatechin-3-gallate from green tea: mechanisms and applications. Food Funct 4:1287-303PubMed View Article
  7.Camfield DA, Stough C, Farrimond J, Scholey AB (2014) Acute effects of tea constituents L-theanine, caffeine, and epigallocatechin gallate on cognitive function and mood: a systematic?review?and meta-analysis. Nutr Rev 72:507-22PubMed View Article
  8.Daemen MA, van’t Veer C, Denecker G, Heemskerk VH, Wolfs TG, Clauss M, Vandenabeele P, Buurman WA (1999) Inhibition of apoptosis induced by ischemia-reperfusion prevents inflammation. J Clin Invest 104:541-49PubMed Central PubMed View Article
  9.Si Y, Bao H, Han L, Shi H, Zhang Y, Xu L, Liu C, Wang J, Vohra A, Ma D (2013) Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. J Transl Med 11:141PubMed Central PubMed View Article
  10.Zhang S, Lv JW, Yang P, Yu Q, Pang J, Wang Z, Guo H, Liu S, Hu J, Li J, Leng J, Huang Y, Ye Z, Wang CY (2012) Loss of dicer exacerbates cyclophosphamide-induced bladder overactivity by enhancing purinergic signaling. Am J Pathol 181:937-46PubMed View Article
  11.Sugiyama A, Chiba M, Nakagami T, Kawano S, Sanada Y, Tajiri T, Toki A (2012) Beneficial effects of (?-epigallocatechin gallate on ischemia-reperfusion?testicular?injury in rats. J Pediatr Surg 47:1427-432PubMed View Article
  12.Kakuta Y, Okumi M, Isaka Y, Tsutahara K, Abe T, Yazawa K, Ichimaru N, Matsumura K, Hyon SH (2011) Epigallocatechin-3-gallate protects kidneys from?ischemia?reperfusion?injury by HO-1 upregulation and inhibition of macrophage infiltration. Transpl Int 24:514-22PubMed View Article
  13.Wang F, Chang Z, Fan Q, Wang L (2014) Epigallocatechin-3-gallate inhibits the proliferation and migration of human ovarian carcinoma cells by modulating?p38?kinase and matrix metalloproteinase-2. Mol Med Rep 9:1085-089PubMed
  14.Li R, Ding T, Liu X, Li C (2006) Influence of SB203580 on cell apoptosis and P38MAPK in?renal?ischemia/reperfusion injury. J Huazhong Univ Sci Technol Med Sci 26:50-2PubMed View Article
  
• 作者单位：Jun Lv (1)
  Min Feng (1)
  LiLi Zhang (1)
  Xia Wan (1)
  Yu Chun Zeng (1)
  Pei Fen Liang (1)
  An Ping Xu (1)
  
  1. Department of Nephrology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Nephrology
  Urology and Andrology
  
• 出版者：Springer Netherlands
• ISSN：1573-2584

文摘

Background Renal ischemia–reperfusion (I/R) injury plays an important role in the acute kidney injury. The pathogenetic mechanisms potential I/R?injury is involved in apoptosis and inflammation. Epigallocatechin gallate (EGCG), a major constituent of green tea, has been shown to have anti-inflammatory and anti-apoptotic activities. This study aimed to explore the underlying effects and mechanisms of EGCG on renal I/R injury in a rat model. Materials and methods We induced renal I/R injury in SD rats by clamping the left renal artery for 45?min followed by 24-h reperfusion, along with a contralateral nephrectomy. We randomly allocated 30 rats to three groups (n?=?10): sham group, IRI group, and EGCG group. We preconditioned rats intraperitoneally with EGCG (50?mg/kg) or vehicle (50?mg/kg) 45?min before inducing renal ischemia. We collected serum and kidneys at 24?h after reperfusion. Renal function and histologic damage were assessed. We also determined markers of inflammation and apoptosis in kidneys or serum. Results EGCG pretreatment can significantly reduce renal dysfunction, histologic change and the expression of tumor necrosis factor-α, IL-1β, IL-6, Bax and cleavage caspase 3 induced by I/R injury and increase the expression of Bax and caspase 3. Moreover, EGCG pretreatment can further induce the activation of p38 mitogen-activated protein kinase in kidney, with no influence on the expression of p38. Conclusions EGCG treatment can decrease renal ischemia–reperfusion injury by suppressing inflammation and cell apoptosis. Thus, EGCG may represent a potential strategy to reduce renal I/R injury.