Genetic Differentiation of the Stingless Bee Tetragonula pagdeni in Thailand Using SSCP Analysis of a Large Subunit of Mitochondrial Ribosomal DNA

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• 作者：Sirikul Thummajitsakul (12) sirikul.thum@gmail.com
  Sirawut Klinbunga (34)
  Siriporn Sittipraneed (1) Siriporn.S@chula.ac.th
• 关键词：Meliponini – ; Tetragonula – ; SSCP – ; Genetic diversity – ; Population differentiation
• 刊名：Biochemical Genetics
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：49
• 期：7-8
• 页码：499-510
• 全文大小：315.2 KB
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• 作者单位：1. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330 Thailand2. Mahidol University, Kanchanaburi Campus, 199 Moo 9 Saiyok District, Kanchanaburi, 71150 Thailand3. Aquatic Molecular Genetics and Biotechnology, Biotec Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120 Thailand4. Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330 Thailand
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Biochemistry
  Zoology
  Medical Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4927

文摘

Genetic diversity and population differentiation of the stingless bee Tetragonula pagdeni (Schwarz) was assessed using single-strand conformational polymorphism (SSCP) analysis of a large subunit of the ribosomal RNA gene (16S rRNA). High levels of genetic variation among individuals within each population (North, Northeast, Central, Prachuap Khiri Khan, Chumphon, and Peninsular Thailand) of T. pagdeni were observed. Analysis of molecular variance indicated significant genetic differentiation among the six geographic populations (Φ _PT = 0.28, P < 0.001) and between samples collected from north and south of the Isthmus of Kra (Φ _PT = 0.18, P < 0.001). In addition, Φ _PT values between all pairwise comparisons were statistically significant (P < 0.01), indicating strong degrees of intraspecific population differentiation. Therefore, PCR-SSCP is a simple and cost-effective technique applicable for routine population genetic analyses in T. pagdeni and other stingless bees. The results also provide an important baseline for the conservation and management of this ecologically important species.