Renoprotective Effect of Humic Acid on Renal Ischemia-Reperfusion Injury: An Experimental Study in Rats

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• 作者：Alpaslan Akbas ; Coskun Silan ; Murat Tolga Gulpinar ; Eyup Burak Sancak…
• 关键词：kidney ; humic acid ; ischemia reperfusion
• 刊名：Inflammation
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：38
• 期：6
• 页码：2042-2048
• 全文大小：790 KB
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• 作者单位：Alpaslan Akbas (1)
  Coskun Silan (2)
  Murat Tolga Gulpinar (1)
  Eyup Burak Sancak (1)
  Sidika Seyma Ozkanli (3)
  Dilek Ulker Cakir (4)
  
  1. Faculty of Medicine, Department of Urology, Canakkale Onsekiz Mart University, Canakkale, Turkey
  2. Faculty of Medicine, Department of Pharmacology, Canakkale Onsekiz Mart University, Canakkale, Turkey
  3. Faculty of Medicine, Department of Pathology, Medeniyet University, Istanbul, Turkey
  4. Faculty of Medicine, Department of Biochemistry, Canakkale Onsekiz Mart University, Canakkale, Turkey
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

Humic acid is an antioxidant molecule used in agriculture and livestock breeding, as well as in medicine. Our aim was to investigate the potential renoprotective effects of humic acid in a renal ischemia reperfusion model. Twenty-one rats were randomly divided into three equal groups. Intraperitoneal serum or humic acid was injected at 1, 12, and 24 h. Non-ischemic group I was evaluated as sham. The left renal artery was clamped in serum (group II) and intraperitoneal humic acid (group III) to subject to left renal ischemic reperfusion procedure. Ischemia and reperfusion time was 60 min for each. Total antioxidant status, total oxidative status, oxidative stress index, and ischemia-modified albumin levels were analyzed biochemically from the serum samples. Kidneys were evaluated histopatologically and immunohistochemically. Biochemical results showed that total oxidative status, ischemia-modified albumin, and oxidative stress index levels were significantly decreased, but total antioxidant status was increased in the humic acid group (III) compared with the ischemia group (II) On histopathological examination, renal tubular dilatation, tubular cell damage and necrosis, dilatation of Bowman’s capsule, hyaline casts, and tubular cell spillage were decreased in the humic acid group (III) compared with the ischemia group (II). Immunohistochemical results showed that apoptosis was deteriorated in group III. Renal ischemia reperfusion injury was attenuated by humic acid administration. These observations indicate that humic acid may have a potential therapeutic effect on renal ischemia reperfusion injury by preventing oxidative stress. KEY WORDS kidney humic acid ischemia reperfusion