Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering

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• 作者：Qian Liu ; Ranran Gao ; Jingen Li ; Liangcai Lin ; Junqi Zhao…
• 关键词：Myceliophthora ; CRISPR/Cas9 system ; Thermophilic fungi ; Cellulases
• 刊名：Biotechnology for Biofuels
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：10
• 期：1
• 全文大小：3834KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
• 出版者：BioMed Central
• ISSN：1754-6834
• 卷排序：10

文摘

BackgroundOver the past 3 years, the CRISPR/Cas9 system has revolutionized the field of genome engineering. However, its application has not yet been validated in thermophilic fungi. Myceliophthora thermophila, an important thermophilic biomass-degrading fungus, has attracted industrial interest for the production of efficient thermostable enzymes. Genetic manipulation of Myceliophthora is crucial for metabolic engineering and to unravel the mechanism of lignocellulose deconstruction. The lack of a powerful, versatile genome-editing tool has impeded the broader exploitation of M. thermophila in biotechnology.