Histological analysis reveals the formation of shoots rather than embryos in regenerating cultures of Eucalyptus globulus

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• 作者：Izabela Dobrowolska ; Gisele M. Andrade…
• 关键词：Eucalyptus globulus ; Histological studies ; In vitro culture ; Regeneration
• 刊名：Plant Cell, Tissue and Organ Culture (PCTOC)
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：128
• 期：2
• 页码：319-326
• 全文大小：2104KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Pathology;
• 出版者：Springer Netherlands
• ISSN：1573-5044
• 卷排序：128

文摘

Eucalyptus globulus is an important species in international forestry in regions with Mediterranean climates and comprises 65 % of the plantation hardwood in Australia. Propagation by somatic embryogenesis would offer many advantages and its development has received much attention. Structures regenerating on explants from hypocotyls of mature zygotic embryos of E. globulus cultured on medium with NAA, reported previously to be effective for embryogenic regeneration, were analyzed morphologically and histologically to clarify their pathway of development. Analysis of series of sections revealed organogenic, rather than embryogenic, pathways of regeneration in this system. We show that protocols for propagation of E. globulus must be carefully evaluated by microscopic examination of adequate numbers of serial sections.