Molecular identification and characterization of prohibitin from Echinococcus granulosus

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• 作者：Xiuqin Zhong ; Xingju Song ; Ning Wang ; Dandan Hu ; Tinayu Liu…
• 关键词：Prohibitin ; Echinococcus granulosus ; Western blotting ; Immunohistochemical localization
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：115
• 期：2
• 页码：897-902
• 全文大小：1,319 KB
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• 作者单位：Xiuqin Zhong (1)
  Xingju Song (1)
  Ning Wang (1)
  Dandan Hu (1)
  Tinayu Liu (1)
  Tao Wang (1)
  Xiaobin Gu (1)
  Weimin Lai (1)
  Xuerong Peng (2)
  Guangyou Yang (1)
  
  1. Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China
  2. Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Prohibitin (PHB) is a widely distributed protein that functions as a molecular chaperone, is involved in the regulation of cell cycle, and maintains mitochondrial structure and functions of the anti-apoptosis, senescence, and proliferation. The aim of this study was to characterize PHB in Echinococcus granulosus (EgPHB), a harmful cestode parasite of humans, many livestock species, and wild animals. We found that EgPHB is a conserved SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing protein, consisting of 289 amino acids, which shares 42.66–99.31 % identity with PHBs from other parasites and mammals. EgPHB was located mainly in the tegument issue of protoscoleces, in the inner body of adult worms, and was expressed widely in the germinal layer. This is the first report on prohibitin from E. granulosus, and EgPHB is considered to be a valuable protein to study more in the future. Key words Prohibitin Echinococcus granulosus Western blotting Immunohistochemical localization