An Ixodes scapularis cell line with a predominantly neuron-like phenotype

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• 作者：Jonathan D. Oliver ; Adela S. Oliva Chávez…
• 关键词：Ixodes scapularis ; Cell culture ; ISE6 ; IDE12 ; Proteome ; Synganglion ; Neuron
• 刊名：Experimental and Applied Acarology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：66
• 期：3
• 页码：427-442
• 全文大小：1,099 KB
• 参考文献：Antwi K, Hanavan PD, Myers CE, Ruiz YW, Thompson EJ, Lake DF (2009) Proteomic identification of an MHC-binding peptidome from pancreas and breast cancer cell lines. Mol Immunol 46:2931-937. doi:10.-016/?j.?molimm.-009.-6.-21 PubMed
  Baldridge GD, Burkhardt NY, Labruna MB, Pacheco RC, Paddock CD, Williamson PC, Billingsley PM, Felsheim RF, Kurtti TJ, Munderloh UG (2010) Wide dispersal and possible multiple origins of low-copy-number plasmids in rickettsia species associated with blood-feeding arthropods. Appl Environ Microbiol 76:1718-731. doi:10.-128/?AEM.-2988-09 PubMed Central PubMed
  Beckmann T, Thüte T, Heinrich C, Büntemeyer H, Noll T (2011) Proteomic and metabolomic characterization of CHO DP-12 cell lines with different high passage histories. BMC Proc 5(Suppl 8):P92. doi:10.-186/-753-6561-5-S8-P92 PubMed Central PubMed
  Bell-Sakyi L, Zweygarth E, Blouin EF, Gould EA, Jongejan F (2007) Tick cell lines: tools for tick and tick-borne disease research. Trends Parasitol 23:450-57. doi:10.-016/?j.?pt.-007.-7.-09 PubMed
  Bell-Sakyi L, Kohl A, Bente DA, Fazakerley JK (2012) Tick cell lines for study of Crimean-Congo hemorrhagic fever virus and other arboviruses. Vector Borne Zoonotic Dis 12:769-81. doi:10.-089/?vbz.-011.-766 PubMed Central PubMed
  Booth TF, Davies CR, Jones LD, Staunton D, Nuttall PA (1989) Anatomical basis of Thogoto virus infection in BHK cell culture and in the ixodid tick vector, Rhipicephalus appendiculatus. J Gen Virol 70(Pt 5):1093-104PubMed
  Chávez ASO, Felsheim RF, Kurtti TJ, Ku P-S, Brayton KA, Munderloh UG (2012) Expression patterns of Anaplasma marginale Msp2 variants change in response to growth in cattle, and tick cells versus mammalian cells. PLoS ONE 7:e36012. doi:10.-371/?journal.?pone.-036012 View Article PubMed
  Chen C, Munderloh UG, Kurtti TJ (1994) Cytogenetic characteristics of cell lines from Ixodes scapularis (Acari: Ixodidae). J Med Entomol 31:425-34PubMed
  Conesa A, G?tz S, García-Gómez JM, Terol J, Talón M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674-676. doi:10.-093/?bioinformatics/?bti610 View Article PubMed
  Egekwu N, Sonenshine DE, Bissinger BW, Roe RM (2014) Transcriptome of the female synganglion of the black-legged tick Ixodes scapularis (Acari: Ixodidae) with comparison between Illumina and 454 systems. PLoS ONE 9:e102667. doi:10.-371/?journal.?pone.-102667 View Article PubMed Central PubMed
  Esteves E, Lara FA, Lorenzini DM, Costa GHN, Fukuzawa AH, Pressinotti LN, Silva JRMC, Ferro JA, Kurtti TJ, Munderloh UG, Daffre S (2008) Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus. Insect Biochem Mol Biol 38:568-80. doi:10.-016/?j.?ibmb.-008.-1.-06 PubMed Central PubMed
  Garcia S, Billecocq A, Crance J-M, Munderloh U, Garin D, Bouloy M (2005) Nairovirus RNA sequences expressed by a Semliki Forest virus replicon induce RNA interference in tick cells. J Virol 79:8942-947. doi:10.-128/?JVI.-9.-4.-942-8947.-005 PubMed Central PubMed
  Intawicha P, Wang S-H, Hsieh Y-C, Lo N-W, Lee K-H, Huang S-Y, Ju J-C (2013) Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS ONE 8:e67772. doi:10.-371/?journal.?pone.-067772 View Article PubMed Central PubMed
  Jenson LJ, Paulson SL, Bloomquist JR (2012) Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents. Invert Neurosci 12:119-27. doi:10.-007/?s10158-012-0138-5 PubMed
  Jouhilahti E-M, Peltonen S, Peltonen J (2008) Class III beta-tubulin is a component of the mitotic spindle in multiple cell types. J Histochem Cytochem 56:1113-119. doi:10.-369/?jhc.-008.-52002 PubMed Central PubMed
  Keller A, Nesvizhskii AI, Kolker E, Aebersold R (2002) Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem 74:5383-392PubMed
  Kim JS, Chang M-Y, Yu IT, Kim JH, Lee S-H, Lee Y-S, Son H (2004) Lithium selectively increases neuronal differentiation of hippocampal neural progenitor cells both in vitro and in vivo. J Neurochem 89:324-36. doi:10.-046/?j.-471-4159.-004.-2329.?x PubMed
  Kuriakose JA, Miyashiro S, Luo T, Zhu B, McBride JW (2011) Ehrlichia chaffeensis transcriptome in mammalian and arthropod hosts reveals differential gene expression and post transcriptional regulation. PLoS ONE 6:e24136. doi:10.-371/?journal.?pone.-024136 View Article PubMed Central PubMed
  Kurtti TJ, Keyhani NO (2008) Intracellular infection of tick cell lines by the entomopathogenic fungus Metarhizium anisopliae. Microbiology 154:1700-709. doi:10.-099/?mic.-.-008/-16667-0 View Article PubMed
  Kurtti TJ, Munderloh UG (1983) The effects of 20-hydroxyecdysone and juvenile hormone III on tick cells. J Para
• 作者单位：Jonathan D. Oliver (1)
  Adela S. Oliva Chávez (2)
  Roderick F. Felsheim (1)
  Timothy J. Kurtti (1)
  Ulrike G. Munderloh (1)
  
  1. Department of Entomology, University of Minnesota, 219 Hodson Hall, St. Paul, MN, 55108, USA
  2. UMR CMAEE, Agricultural Research Centre for International Development - Control of Animal Diseases, Emerging and Exotic, CIRAD, Site de Duclos, Prise d’eau, 97170, Petit-Bourg, Guadeloupe, France
  
• 刊物主题：Entomology; Animal Systematics/Taxonomy/Biogeography; Animal Genetics and Genomics; Animal Ecology; Life Sciences, general;
• 出版者：Springer Netherlands
• ISSN：1572-9702

文摘

The Ixodes scapularis embryo-derived cell line ISE6 is the most widely utilized tick-derived cell line due to its susceptibility to a wide variety of tick- and non-tick-vectored pathogens. Little is known about its tissue origin or biological background. Protein expression of ISE6 cells was compared with that of another I. scapularis-derived cell line, IDE12, and dissected tick synganglia. Results demonstrated the presence of a neuronal marker protein, type 3 β-tubulin, in all three samples, as well as other shared and unique neuronal and immune response-associated proteins. Of neuronal proteins shared between the two cell lines, ISE6 expressed several in significantly greater quantities than IDE12. Stimulation of ISE6 cells by in vivo exposure to the hemocoel environment in unfed larval and molting nymphal ticks, but not unfed nymphal ticks, resulted in the development of neuron-like morphologic characteristics in the implanted cells.