Cow dung: a potential biomass substrate for the production of detergent-stable dehairing protease by alkaliphilic Bacillus subtilis strain VV

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• 作者：Ponnuswamy Vijayaraghavan ; Aija Vijayan ; Arumugaperumal Arun…
• 关键词：Cow dung ; Solid ; state fermentation ; Bacillus subtilis strain VV ; Alkaline protease ; Detergent ; stable
• 刊名：SpringerPlus
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：1
• 期：1
• 全文大小：623KB
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• 作者单位：Ponnuswamy Vijayaraghavan (1)
  Aija Vijayan (2)
  Arumugaperumal Arun (3)
  John Kennady Jenisha (3)
  Samuel Gnana Prakash Vincent (1)
  
  1. International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam鈥?29 502, Kanyakumari District, Tamil Nadu, India
  2. Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam鈥?29 502, Kanyakumari District, Tamil Nadu, India
  3. Department of Biotechnology, Kalasalingam University, Virudhunagar, Srivilliputtur, 626 126, Tamilnadu, India
  
• 刊物类别：Science, general;
• 刊物主题：Science, general;
• 出版者：Springer International Publishing
• ISSN：2193-1801

文摘

Cow dung, a cheap and easily available source of energy, was used as the substrate for the production of alkaline protease by solid-state fermentation using the Bacillus subtilis strain VV. In order to achieve the maximum yield of this enzyme, the following optimum process parameters are needed: fermentation period (72 h), pH (10.0), moisture content (140%), inoculum (25%), temperature (30鈥?0掳C), carbon source (2% (w/w) maltose) and nitrogen source (1% (w/w) urea). The protease was stable over a broad temperature range (30鈥?0掳C) and pH (8.0-10.0), with maximum activity at 50掳C and pH 10.0. Among the divalent ions tested, Ca2+ (0.01 M) increased enzyme activity. The purified protease, after being subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis, was found to have a molecular mass of 38.5 kDa. The enzyme was solvent-and surfactant-stable and showed activity even after 24 h incubation along with various commercially available detergents. This enzyme possessed dehairing properties for animal hide after 16 h of incubation at room temperature. From these results it is evident that cow dung is a potential substrate for the production of a detergent-stable, dehairing protease by B. subtilis. This enzyme has a lot of potential applications in the detergent and leather-processing industries. Keywords Cow dung Solid-state fermentation Bacillus subtilis strain VV Alkaline protease Detergent-stable