Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage

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• 作者：Marie JJ Huysman (1) (2) (3) (4)
  Atsuko Tanaka (4) (5)
  Chris Bowler (4)
  Wim Vyverman (3)
  Lieven De Veylder (1) (2)
  
  1. Department of Plant Systems Biology ; Flanders Institute for Biotechnology (VIB) ; 9052 ; Ghent ; Belgium
  2. Department of Plant Systems Biology ; VIB ; and Bioinformatics ; Ghent University ; 9052 ; Ghent ; Belgium
  3. Protistology and Aquatic Ecology ; Department of Biology ; Ghent University ; 9000 ; Ghent ; Belgium
  4. Environmental and Evolutionary Genomics Section ; Institut de Biologie de l鈥橢cole Normale Sup茅rieure ; Centre National de la Recherche Scientifique ; Unit茅 Mixte de Recherche 8186 ; Institut National de la Sant茅 et de la Recherche M茅dicale U1024 ; Ecole Normale Sup茅rieure ; 75230 ; Paris ; Cedex 05 ; France
  5. Current address ; Muroran Marine Station ; Field Science Center for Northern Biosphere ; Hokkaido University ; Muroran ; Hokkaido ; 051-0013 ; Japan
  
• 关键词：Cyclin ; dependent kinase ; CDKA2 ; Cell cycle ; Cell division ; Cytokinesis ; Diatom ; Mitosis ; Phaeodactylum tricornutum
• 刊名：BMC Plant Biology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：1,600 KB
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• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background Cyclin-dependent kinases (CDKs) are crucial regulators of cell cycle progression in eukaryotes. The diatom CDKA2 was originally assigned to the classical A-type CDKs, but its cell cycle phase-specific transcription at the G2-to-M phase transition is typical for plant-specific B-type CDKs. Results Here, we report the functional characterization of CDKA2 from the diatom Phaeodactylum tricornutum. Through a yeast two-hybrid library screen, CDKA2 was found to interact with the G2/M-specific CDK scaffolding factor CKS1. Localization of CDKA2 was found to be nuclear in interphase cells, while in cells undergoing cytokinesis, the signal extended to the cell division plane. In addition, overexpression of CDKA2 induced an overall reduction in the cell growth rate. Expression analysis of cell cycle marker genes in the overexpression lines indicates that this growth reduction is primarily due to a prolongation of the mitotic phase. Conclusions Our study indicates a role for CDKA2 during cell division in diatoms. The functional characterization of a CDK with clear CDKB properties in a non-green organism questions whether the current definition of B-type CDKs being plant-specific might need revision.