Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries

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• 作者：Barindra Sana ; Kuan Hui Burton Chia ; Sarada S. Raghavan…
• 关键词：Directed evolution ; Fluorescence ; activated cell sorting ; RNA sequencing ; Lignin ; Vanillin ; Enzyme engineering ; Biorefinery ; Vanillin ; inducible promoter ; Microbial biosensor ; High ; throughput screening
• 刊名：Biotechnology for Biofuels
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：10
• 期：1
• 全文大小：3931KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
• 出版者：BioMed Central
• ISSN：1754-6834
• 卷排序：10

文摘

Background Lignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount of lignin is produced as a by-product of the paper industry, while cellulosic components of plant biomass are utilized for the production of paper pulp. In spite of vast potential, lignin remains the least exploited component of plant biomass due to its extremely complex and heterogenous structure. Several enzymes have been reported to have lignin-degrading properties and could be potentially used in lignin biorefining if their catalytic properties could be improved by enzyme engineering. The much needed improvement of lignin-degrading enzymes by high-throughput selection techniques such as directed evolution is currently limited, as robust methods for detecting the conversion of lignin to desired small molecules are not available.