A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO₂ nanosheets and copper nanoclusters

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• 作者：Hai-Bo Wang ; Ying Chen ; Na Li ; Yan-Ming Liu
• 关键词：Fluorescent bioassay ; Nanoprobes ; Glucose detection ; Glucose oxidase ; Enzymatically generated H2O2 ; Glucuronic acid ; Mn(II) ions
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：184
• 期：2
• 页码：515-523
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

The authors describe a fluorometric glucose assay that is based on the use of MnO₂ nanosheets and copper nanoclusters (CuNCs) acting as nanoprobes. The CuNCs were synthesized by using bovine serum albumin as a template by chemical reduction of copper(II) sulfate. On addition of MnO₂ nanosheets to a colloidal solution of CuNCs, the fluorescence of CuNCs (measured at excitation/emission wavelengths of 335/410 nm) is quenched. However, in the presence of enzymatically generated H₂O₂, the MnO₂ nanosheets are reduced to form Mn(II) ions. As a result, fluorescence intensity recovers. The glucose assay is based on the enzymatic conversion of glucose by glucose oxidase to generate H₂O₂ and glucuronic acid. The calibration plot is linear in the 1 μM to 200 μM glucose concentration range, and the detection limit is 100 nM. The method was successfully applied to the determination of glucose in spiked human serum samples.