Proteomic analysis of the extraembryonic tissues from cloned porcine fetus at day 35 of pregnancy

详细信息    查看全文

• 作者：Yeoung-Gyu Ko (1)
  Seongsoo Hwang (1)
  Sung Woo Kim (1)
  Hyun Kim (1)
  Hwan Hoo Seong (1)
  Jae-Hwan Kim (1)
  Yuno Song (2)
  Bo-Suck Yang (3)
  Young Min Song (3)
  Jae-Hyeon Cho (2)
  
  1. Animal Genetic Resources Station ; National Institute of Animal Science ; RDA ; Namwon ; 590-832 ; Korea
  2. Institute of Agriculture and Life Science ; College of Veterinary Medicine ; Gyeongsang National University ; Jiju Daero 501 ; Jinju ; Gyeongsangnamdo ; 660-701 ; Korea
  3. Department of Animal Science & Biotechnology ; Gyeongnam National University of Science and Technology ; Jinju ; 660-758 ; Korea
  
• 关键词：Extraembryonic tissue ; Placenta ; SCNT ; Apotosis ; Gene expression
• 刊名：BMC Research Notes
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：1,175 KB
• 参考文献：1. Rogatcheva, MM, Rund, LA, Swanson, KS, Marron, BM, Beever, JE, Counter, CM, Schook, LB (2004) Creating porcine biomedical models through recombineering. Comp Funct Genomics 5: pp. 262-267 CrossRef
  2. Fujimura, T, Takahagi, Y, Shigehisa, T, Nagashima, H, Miyagawa, S, Shirakura, R, Murakami, H (2008) Production of alpha 1,3-galactosyltransferase gene-deficient pigs by somatic cell nuclear transfer: a novel selection method for gal alpha 1,3-Gal antigen-deficient cells. Mol Reprod Dev 75: pp. 1372-1378 CrossRef
  3. Onishi, A, Iwamoto, M, Akita, T, Mikawa, S, Takeda, K, Awata, T, Hanada, H, Perry, AC (2000) Pig cloning by microinjection of fetal fibroblast nuclei. Science 289: pp. 1188-1190 CrossRef
  4. Polejaeva, IA, Chen, SH, Vaught, TD, Page, RL, Mullins, J, Ball, S, Dai, Y, Boone, J, Walker, S, Ayares, DL, Colman, A, Campbell, KH (2000) Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 7: pp. 86-90 CrossRef
  5. Hashizume, K, Ishiwata, H, Kizaki, K, Yamada, O, Takahashi, T, Imai, K, Patel, OV, Akagi, S, Shimizu, M, Takahashi, S, Katsuma, S, Shiojima, S, Hirasawa, A, Tsujimoto, G, Todoroki, J, Izaike, Y (2002) Implantation and placental development in somatic cell clone recipient cows. Clon Stem Cell 4: pp. 197-209 CrossRef
  6. Hill, JR, Burghardt, RC, Jones, K, Long, CR, Looney, CR, Shin, T, Spencer, TE, Thompson, JA, Winger, QA, Westhusin, ME (2000) Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses. Biol Reprod 63: pp. 1787-1794 CrossRef
  7. Hill, JR, Edwards, JF, Sawyer, N, Blackwell, C, Cibelli, JB (2001) Placental anomalies in a viable cloned calf. Cloning 3: pp. 83-88 CrossRef
  8. Chae, JI, Yu, K, Cho, SK, Kim, JH, Koo, DB, Lee, KK, Han, YM (2008) Aberrant expression of developmentally important signaling molecules in cloned porcine extraembrynic tissues. Proteomics 8: pp. 2724-2734 CrossRef
  9. Bauersachs, S, Ulbrich, SE, Zakhartchenko, V, Minten, M, Reichenbach, M, Reichenbach, HD, Blum, H, Spencer, TE, Wolf, E (2009) The endometrium responds differently to cloned versus fertilized embryos. Proc Natl Acad Sci U S A 106: pp. 5681-5686 CrossRef
  10. Humpherys, D, Eggan, K, Akutsu, H, Friedman, A, Hochedlinger, K, Yanagimachi, R, Lander, ES, Golub, TR, Jaenisch, R (2002) Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei. Proc Natl Acad Sci U S A 99: pp. 12889-12894 CrossRef
  11. Cho, SK, Kim, JH, Park, JY, Choi, YJ, Bang, JI, Hwang, KC, Cho, EJ, Sohn, SH, Uhm, SJ, Koo, DB, Lee, KK, Kim, T, Kim, JH (2007) Serial cloning of pigs by somatic cell nuclear transfer: restoration of phenotypic normality during serial cloning. Dev Dyn 236: pp. 3369-3382 CrossRef
  12. Smith, SC, Baker, PN, Symonds, EM (1997) Placental apoptosis in normal human pregnancy. Am J Obstet Gynecol 177: pp. 57-65 CrossRef
  13. Wisdom, SJ, Wilson, R, McKillop, JH, Walker, JJ (1991) Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension. Am J Obstet Gynecol 165: pp. 1701-1704 CrossRef
  14. Chae, JI, Lee, KS, Kim, DJ, Han, YM, Lee, DS, Lee, KK, Koo, DB (2009) Abnormal gene expression in extraembryonic tissue from cloned porcine embryos. Theriogenology 71: pp. 323-333 CrossRef
  15. Constant, F, Guillomot, M, Heyman, Y, Vignon, X, Laigre, P, Servely, JL, Renard, JP, Chavatte-Palmer, P (2006) Large offspring or large placenta syndrome? Morphometric analysis of late gestation bovine placentomes from somatic nuclear transfer pregnancies complicated by hydrallantois. Biol Reprod 75: pp. 122-130 CrossRef
  16. Strickland, S, Richards, WG (1992) Invasion of the trophoblasts. Cell 71: pp. 355-357 CrossRef
  17. Tanaka, S, Oda, M, Toyoshima, Y, Wakayama, T, Tanaka, M, Yoshida, N, Hattori, N, Ohgane, J, Yanagimachi, R, Shiota, K (2001) Placentomegaly in cloned mouse concepti caused by expansion of the spongiotrophoblast layer. Biol Reprod 65: pp. 1813-1821 CrossRef
  18. Chavatte-Palmer, P, Camous, S, Jammes, H, Le Cleac鈥檋, N, Guillomot, M, Leed, RSF (2012) Placental perturbations induce the developmental abnormalities often observed in bovine somatic cell nuclear transfer. Placenta 26: pp. 99-104 CrossRef
  19. Beyhan, Z, Ross, PJ, Iager, AE, Kocabas, AM, Cunniff, K, Rosa, GJ, Cibelli, JB (2007) Transcriptional reprogramming of somatic cell nuclei during preimplantation development of cloned bovine embryos. Dev Biol 305: pp. 637-649 CrossRef
  20. Nolen, LD, Gao, S, Han, Z, Mann, MR, Chung, Y, Otte, AP, Bartolomei, MS, Latham, KE (2005) X chromosome reactivation and regulation in cloned embryos. Dev Biol 279: pp. 525-540 CrossRef
  21. Dean, W, Santos, F, Stojkovic, M, Zakhartchenko, V, Walter, J, Wolf, E, Reik, W (2001) Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. Proc Natl Acad Sci U S A 98: pp. 13734-13738 CrossRef
  22. Kang, YK, Koo, DB, Park, JS, Choi, YH, Chung, AS, Lee, KK, Han, YM (2001) Aberrant methylation of donor genome in cloned bovine embryos. Nat Genet 28: pp. 173-177 CrossRef
  23. Hussain, SP, Amstad, P, He, P, Robles, A, Lupold, S, Kaneko, I, Ichimiya, M, Sengupta, S, Mechanic, L, Okamura, S, Hofseth, LJ, Moake, M, Nagashima, M, Forrester, KS, Harris, CC (2004) p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis. Cancer Res 64: pp. 2350-2356 CrossRef
  24. Chae, JI, Cho, SK, Seo, JW, Yoon, TS, Lee, KS, Kim, JH, Lee, KK, Han, YM, Yu, K (2006) Proteomic analysis of the extraembryonic tissue from cloned porcine embryos. Mol Cell Proteomics 5: pp. 1559-1566 CrossRef
  25. Concannon, CG, Gorman, AM, Samali, A (2003) On the role of Hsp27 in regulating apoptosis. Apoptosis 8: pp. 61-70 CrossRef
  26. Park, MR, Im, GS, Kim, SW, Hwang, S, Park, JH, Kim, H, Do, YJ, Park, SB, Yang, BS, Song, YM, Cho, JH, Ko, YG (2013) Aberrant gene expression patterns in extraembryonic tissue from cloned porcine embryos. Res Vet Sci 94: pp. 531-538 CrossRef
  
• 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500

文摘

Background Somatic cell cloning by nuclear transfer (SCNT) in pig is clearly of great benefit for basic research and biomedical applications. Even though cloned offspring have been successfully produced in pig, SCNT is struggling with the low efficiency. Results In the present study, we investigated differentially expressed proteins of the extraembryonic tissue from pig SCNT fetus compared to control (normal) fetus. We obtained the extraembryonic tissue from embryos at day 35 of pregnancy and examined the protein expression profiles using two-dimensional electrophoresis (2-D) and Western blotting. The extraembryonic tissue of fetus in control pregnancy was compared to the extraembryonic tissue of SCNT fetus, which showed an abnormally small size and shape as well as exhibited abnormal placental morphology compared to control fetus. A proteomic analysis showed that the expression of 33 proteins was significantly increased or decreased in the extraembryonic tissue of SCNT fetus compared to control fetus. The differentially expressed proteins in the extraembryonic tissue of SCNT fetus included ATP or lipid binding proteins, antioxidant proteins, translation elongation factors, and transcription factors. Western blotting analysis indicated that antioxidant enzymes and anti-apoptotic proteins were down-regulated; however, the expression levels of apoptotic proteins, Bax and Hsp27, were increased in the extraembryonic tissue of SCNT fetus. Moreover, immunohistochemical analysis also showed that the expression of the catalase or GPX genes was decreased in the extraembryonic tissue with SCNT fetus compared to those with control fetus. In addition, we observed a significant decrease in DNA methytransferase1 (Dnmt1) expression in SCNT extraembryonic tissue, and the expression levels of Dnmt3a and Dnmt3b were abnormally higher in SCNT fetus compared to control fetus. Moreover, a marked increase in the frequency of TUNEL-positive cells was observed in the extraembryonic tissue in SCNT fetus. Conclusion These results demonstrated that pig SCNT fetus showed abnormal protein expression in the extraembryonic tissue, and extensive apoptosis occurred in the extraembryonic tissue of the SCNT fetus due to an increase in apoptotic protein expression or a decrease in antioxidant protein expression.