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Urinary ATP as an indicator of infection and inflammation of the urinary tract in patients with lower urinary tract symptoms
- 作者:Kiren Gill ; Harry Horsley ; Anthony S Kupelian ; Gianluca Baio ; Maria De Iorio…
- 关键词:Lower urinary tract symptoms (LUTS) ; Adenosine ; 5-triphosphate (ATP) ; Urinary tract infection (UTI)
- 刊名:BMC Urology
- 出版年:2015
- 出版时间:December 2015
- 年:2015
- 卷:15
- 期:1
- 全文大小:663 KB
- 参考文献:1. Coyne, KS, Sexton, CC, Thompson, CL, Milsom, I, Irwin, D, Kopp, ZS (2009) The prevalence of lower urinary tract symptoms (LUTS) in the USA, the UK and Sweden: results from the Epidemiology of LUTS (EpiLUTS) study. B J UInt 104: pp. 352-60
2. Staskin, D, Kelleher, C, Bosch, R, Cotterill, N, Emmanuel, A, Yoshida, M Initial Assessment of Urinary and Faecal Incontinence in Adult Male and Female Patients. In: class="editors">Abrams, P, Cardozo, L, Khoury, S, Wein, A eds. (2009) 4th International Conusltation on Incontinence. International Conusltation on Incontinence. Health Publications Ltd, Paris, pp. 333-62 3. Khasriya, R, Khan, S, Lunawat, R, Bishara, S, Bignal, J, Malone-Lee, M (2010) The Inadequacy of Urinary Dipstick and Microscopy as Surrogate Markers of Urinary Tract Infection in Urological Outpatients With Lower Urinary Tract Symptoms Without Acute Frequency and Dysuria. J Urol 183: pp. 1843-7 class="external" href="http://dx.doi.org/10.1016/j.juro.2010.01.008" target="_blank" title="It opens in new window">CrossRef 4. Deville, WL, Yzermans, JC, Duijn, NP, Bezemer, PD, Windt, DA, Bouter, LM (2004) The urine dipstick test useful to rule out infections. A meta-analysis of the accuracy. BMC Urol 4: pp. 4 class="external" href="http://dx.doi.org/10.1186/1471-2490-4-4" target="_blank" title="It opens in new window">CrossRef 5. Gorelick, MH, Shaw, KN (1999) Screening tests for urinary tract infection in children: A meta-analysis. Pediatrics 104: pp. e54 class="external" href="http://dx.doi.org/10.1542/peds.104.5.e54" target="_blank" title="It opens in new window">CrossRef 6. Stamm, WE, Counts, GW, Running, KR, Fihn, S, Turck, M, Holmes, KK (1982) Diagnosis of coliform infection in acutely dysuric women. N Engl J Med 307: pp. 463-8 class="external" href="http://dx.doi.org/10.1056/NEJM198208193070802" target="_blank" title="It opens in new window">CrossRef 7. Kass, EH (1957) Bacteriuria and the diagnosis of infection in the urinary tract. Arch Intern Med 100: pp. 709-14 class="external" href="http://dx.doi.org/10.1001/archinte.1957.00260110025004" target="_blank" title="It opens in new window">CrossRef 8. Siegman-Igra, Y (1994) The significance of urine culture with mixed flora. Curr Opin Nephrol Hypertens 3: pp. 656-9 class="external" href="http://dx.doi.org/10.1097/00041552-199411000-00017" target="_blank" title="It opens in new window">CrossRef 9. Hurlbut, TA, Littenberg, B (1991) The diagnostic accuracy of rapid dipstick tests to predict urinary tract infection. Am J Clin Pathol 96: pp. 582-8 10. Burnstock, G (2011) Therapeutic potential of purinergic signalling for diseases of the urinary tract. BJU Int 107: pp. 192-204 class="external" href="http://dx.doi.org/10.1111/j.1464-410X.2010.09926.x" target="_blank" title="It opens in new window">CrossRef 11. Wijk, T, Jonge, HR, Tilly, BC (1999) Osmotic cell swelling-induced ATP release mediates the activation of extracellular signal-regulated protein kinase (Erk)-1/2 but not the activation of osmo-sensitive anion channels. Biochem J 343: pp. 579-86 class="external" href="http://dx.doi.org/10.1042/0264-6021:3430579" target="_blank" title="It opens in new window">CrossRef 12. Weyden, L, Conigrave, AD, Morris, MB (2000) Signal transduction and white cell maturation via extracellular ATP and the P2Y11 receptor. Immunol Cell Biol 78: pp. 369-74 class="external" href="http://dx.doi.org/10.1046/j.1440-1711.2000.00918.x" target="_blank" title="It opens in new window">CrossRef 13. Lundin, A, Hallander, H, Kallner, A, Lundin, UK, Osterberg, E (1989) Bacteriuria testing by the ATP method as an integral part in the diagnosis and therapy of urinary tract infection (UTI). J Biolumin Chemilumin 4: pp. 381-9 class="external" href="http://dx.doi.org/10.1002/bio.1170040152" target="_blank" title="It opens in new window">CrossRef 14. Save, S, Persson, K (2010) Extracellular ATP and P2Y receptor activation induce a proinflammatory host response in the human urinary tract. Infect Immun 78: pp. 3609-15 class="external" href="http://dx.doi.org/10.1128/IAI.00074-10" target="_blank" title="It opens in new window">CrossRef 15. Hanberger, H, Nilsson, LE, Kihlstrom, E, Maller, R (1990) Postantibiotic effect of beta-lactam antibiotics on Escherichia coli evaluated by bioluminescence assay of bacterial ATP. Antimicrob Agents Chemother 34: pp. 102-6 class="external" href="http://dx.doi.org/10.1128/AAC.34.1.102" target="_blank" title="It opens in new window">CrossRef 16. Poulis, JA, Pijper, M, Mossel, DA, Dekkers, PP (1993) Assessment of cleaning and disinfection in the food industry with the rapid ATP-bioluminescence technique combined with the tissue fluid contamination test and a conventional microbiological method. Int J Food Microbiol 20: pp. 109-16 class="external" href="http://dx.doi.org/10.1016/0168-1605(93)90098-2" - 刊物主题:Urology/Andrology; Internal Medicine;
- 出版者:BioMed Central
- ISSN:1471-2490
文摘
Background Adenosine-5-triphosphate (ATP) is a neurotransmitter and inflammatory cytokine implicated in the pathophysiology of lower urinary tract disease. ATP additionally reflects microbial biomass thus has potential as a surrogate marker of urinary tract infection (UTI). The optimum clinical sampling method for ATP urinalysis has not been established. We tested the potential of urinary ATP in the assessment of lower urinary tract symptoms, infection and inflammation, and validated sampling methods for clinical practice. Methods A prospective, blinded, cross-sectional observational study of adult patients presenting with lower urinary tract symptoms (LUTS) and asymptomatic controls, was conducted between October 2009 and October 2012. Urinary ATP was assayed by a luciferin-luciferase method, pyuria counted by microscopy of fresh unspun urine and symptoms assessed using validated questionnaires. The sample collection, storage and processing methods were also validated. Results 75 controls and 340 patients with LUTS were grouped as without pyuria (n--00), pyuria 1-9 wbc μl-1 (n--20) and pyuria ?0 wbc μl-1 (n--20). Urinary ATP was higher in association with female gender, voiding symptoms, pyuria greater than 10 wbc μl-1 and negative MSU culture. ROC curve analysis showed no evidence of diagnostic test potential. The urinary ATP signal decayed with storage at 23°C but was prevented by immediate freezing at?≤-20°C, without boric acid preservative and without the need to centrifuge urine prior to freezing. Conclusions Urinary ATP may have a role as a research tool but is unconvincing as a surrogate, clinical diagnostic marker.
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