Detection of vibrio cholerae O1 by using cerium oxide nanowires - based immunosensor with different antibody immobilization methods

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• 作者：Phuong Dinh Tam ; Nguyen Luong Hoang ; Hoang Lan…
• 刊名：Journal of the Korean Physical Society
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：68
• 期：10
• 页码：1235-1245
• 全文大小：2,114 KB
• 刊物主题：Physics, general; Theoretical, Mathematical and Computational Physics; Particle and Nuclear Physics;
• 出版者：Springer Netherlands
• ISSN：1976-8524
• 卷排序：68

文摘

In this work, we evaluated the effects of different antibody immobilization strategies on the response of a CeO₂-nanowires (NWs)-based immunosensor for Vibrio cholerae O1 detection. Accordingly, the changes in the electron-transfer resistance (R_et) from before to after cells bind to an antibody-modified electrode prepared by using three different methods of antibody immobilization were determined. The values were 16.2%, 8.3%, and 6.65% for the method that utilized protein A, antibodies activated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS), and absorption, respectively. Cyclic voltammetry confirmed that the change in the current was highest for the immunosensors prepared using protein A (11%), followed by those prepared with EDC/NHS-activated antibodies (9%), and finally, those prepared through absorption (7.5%). The order of the antibody immobilization strategies in terms of resulting immunosensor detection limit and sensitivity was as follows order: absorption (3.2 × 103 CFU/mL; 45.1 Ω/CFU·mL−1) < EDC/NHS-activated antibody (1.0 × 103 CFU/mL; 50.6 Ω/CFU·mL−1) < protein A (1.0 × 102 CFU/mL; 65.8 Ω/CFU·mL−1). Thus, we confirmed that the protein A - mediated method showed significantly high cell binding efficiencies compared to the random immobilization method.KeywordsAntibodyProtein AImmunosensorNanowireCeO₂