Cryopreservation for retaining morphology, genetic integrity, and foreign genes in transgenic plants of Torenia fournieri

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• 作者：Jing-Wei Li ; Hou-Hua Li ; Ren-Rui Wang ; Xiao-Xia Gao…
• 关键词：Cry 1Ab ; Droplet ; vitrification cryopreservation ; Genetic integrity ; Morphology ; Shoot tips ; Torenia fournieri
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：38
• 期：1
• 全文大小：3,944 KB
• 参考文献：Aida R (2008) Torenia fournieri (torenia) as a model plant for transgenic studies. Plant Biotechnol 25:541–545CrossRef
  Aida R, Kishimotoa S, Tanaka Y, Shibata M (2000) Modification of flower color in torenia (Torenia fournieri Lind.) by genetic transformation. Plant Sci 153:33–42CrossRef
  Benson EE (2008) Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Crit Rev Plant Sci 27:141–219CrossRef
  Cao J, Earle ED (2003) Transgene expression in broccoli (Brassica oleracea var. italica) clones propagated in vitro via leaf explants. Plant Cell Rep 21:789–796PubMed
  Chandler SF, Sanchez C (2012) Genetic modification; the development of transgenic ornamental plant varieties. Plant Biotechnol J 10:891–903CrossRef PubMed
  Cheong EJ (2012) Biotechnological approaches for improvement and conservation of Prunus species. Plant Biotechnnol Rep 6:17–28CrossRef
  Cho JS, Hong SM, Joo SY, Yoo JS, Kim DI (2007) Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig. Appl Microbiol Biotechnol 73:1470–1476CrossRef PubMed
  Cornejo MJ, Wong VL, Blechl AE (1995) Cryopreserved callus: a source of protoplasts for rice transformation. Plant Cell Rep 14:210–214CrossRef PubMed
  Corredoira E, San-José MC, Vieitez AM, Ballester A (2007) Improving genetic transformation of European chestnut and cryopreservation of transgenic lines. Plant Cell Tissue Org Cult 91:281–288CrossRef
  Elleuch H, Gazeau C, David H, David A (1998) Cryopreservation does not affect the expression of a foreign sam gene in transgenic Papaver somniferum cells. Plant Cell Rep 18:94–98CrossRef
  Fretz A, Lorz H (1995) Cryopreservation of in vitro cultures of barley (Hordeum vulgare L. and H. murinum L.) and transgenic cells of wheat (Triticum aestivum L.). J Plant Physiol 146:489–496CrossRef
  Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051CrossRef PubMed
  Gazeau C, Elleuch H, David A, Morisset C (1998) Cryopreservation of transformed Papaver somniferum cells. CryoLett 19:147–158
  Hao YJ, Deng XX (2003) GUS gene remains stable in transgenic Citrus callus recovered from cryopreservation. CryoLett 24:375–380
  Hao YJ, Cheng YJ, Deng XX (2005) Stable maintenance and expression of a foreign gene in transgenic pear shoots retrieved from in vitro conservation. J Plant Physiol 162:237–243CrossRef PubMed
  Harding K (2004) Genetic integrity of cryopreserved plant cells: a review. CryoLett 25:3–22
  Hitmi A, Barthomeuf Ch, Sallanon H (2000) Cryopreservation of Chrysanthemum cinerariaefolium shoot tips. J Plant Physiol 156:408–412CrossRef
  Hornung R, Holland A, Taylor HF, Lynch PT (2001) Cryopreservation of auricula shoot tips using the encapsulation/dehydration technique. CryoLett 22:27–34
  Ishikawa K, Harata K, Mii M, Sakai A, Yoshimatsu K, Shimomura K (1997) Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification. Plant Cell Rep 16:754–757CrossRef
  James C (2010) A global overview of biotech (GM) crops: adoption, impact and future prospects GM Crops. GM Crop 1:8–12CrossRef
  Kulus D, Zalewska M (2014) Cryopreservation as a tool used in long-term storage of ornamental species—a review. Sci Hortic 168:88–107CrossRef
  Li HH, Zhao B, Seitz C, Forkmann G (2009) In vitro plant regeneration and Agrobacterium-mediated genetic transformation of Torenia fournieri. In: Zhang QX (ed) Advances in ornamental horticulture. Forest Press of China, Beijing, pp 183–187
  Li ZA, Du YQ, Wang ZC (2013) Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana. Electron J Biotechnol 16:6. doi:10.​2225/​vol16-issue6-fulltext-12
  Meijer EGM, Iren E, Schrijnemakers E, Hensgens LAM, van Zijderveld M, Schilperoort RA (1991) Retention of the capacity to produce plants from protoplasts in cryopreserved cell lines of rice (Oryza sativa L). Plant Cell Rep 10:171–174CrossRef PubMed
  Menges M, Murray AH (2004) Cryopreservation of transformed and wild-type Arabidopsis and tobacco cell suspension cultures. Plant J 37:635–644CrossRef PubMed
  Mezzalama M, Crouch JH, Ortiz R (2010) Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials. Electron J Biotechnol 13:2. doi:10.​2225/​vol13-issue2-fulltext-5 . http://​www.​ejbiotechnology.​info/​content/​vol13/​issue2/​full/​5/​
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cell cultures. Physiol Plant 15:473–497CrossRef
  Na HY, Kondo K (1996) Cryopreservation of tissue-cultured shoot primordia from shoot apices of cultured protocorms in Vanda pumila following ABA preculture and desiccation. Plant Sci 118:195–201CrossRef
  Ogawa Y, Suzuki H, Sakurai N, Aoki K, Saito K, Shibata D (2008) Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells. CryoLett 29:427–436
  Perlak FJ, Fuchs RL, Dean DA, Mcpherson SL, Fischhoff DA (1991) Modification of the coding sequence enhances plant expression of insect control protein genes (Bacillus thuringiensis/insect-resistant plants/synthetic genes). Proc Nat Acad Sci 88:3324–3328PubMedCentral CrossRef PubMed
  Ryynänen L, Sillanpää M, Kontunen-Soppela S, Tiimonen H, Kangasjärvi J, Vapaavuori E (2002) Preservation of transgenic silver birch (Betula pendula Roth) lines by means of cryopreservation. Mol Breed 10:143–152CrossRef
  Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33CrossRef PubMed
  Sansavini S, Belfanti E, Costa F, Donati F (2005) European apple breeding programs turn to biotechnology. Chron Hortic 45:16–19
  Schmale K, Rademacher TH, Fischer R, Hellwig S (2006) Towards industrial usefulness—cryocell-banking of transgenic BY-2 cell cultures. J Biotechnol 124:302–311CrossRef PubMed
  Sopalun K, Kanchit K, Ishikawa K (2010) Vitrification-based cryopreservation of Grammatophyllum speciosum protocorm. CryoLett 31:347–357
  Suzuki M, Akihama T, Ishikawa M (2005) Cryopreservation of encapsulated gentian axillary buds following 2 step-preculture with sucrose and desiccation. Plant Cell Tissue Org Cult 83:115–121CrossRef
  Teixeira da Silva JA (2004) Ornamental chrysanthemums: improvement by biotechnology. Plant Cell Tissue Org Cult 79:1–18CrossRef
  Tsai SF, Yeh SD, Chan CF, Liaw SL (2009) High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of transgenic papaya lines. Plant Cell Tissue Org Cult 98:157–164CrossRef
  Valenzuela S, Balocchi C, Rodríguez J (2006) Transgenic trees and forestry biosafety. Electron J Biotechnol. http://​www.​ejbiotechnology.​info/​content/​vol9/​issue3/​full/​22/​
  Van Eck J, Keen P (2009) Continued expression of plant-made vaccines following long-term cryopreservation of antigen-expressing tobacco cell cultures. In Vitro Cell Dev Biol Plant 45:750–757CrossRef
  Wang F, Yuan QH, Shi L, Qian Q, Liu WG, Kuang BG, Zeng DL, Liao YL, Cao B, Jia SR (2006) A large-scale field study of transgene flow from cultivated rice (Oryza sativa) to common wild rice (O. rufipogon) and barnyard grass (Echinochloa crusgalli). Plant Biotechnol J 4:667–676CrossRef PubMed
  Wang B, Zhang ZB, Yin ZF, Feng CH, Wang QC (2012a) Novel and potential application of cryopreservation to plant genetic transformation. Biotechnol Adv 30:604–612CrossRef PubMed
  Wang QC, Wang RR, Li BQ, Cui ZH (2012b) Cryopreservation: a strategy for safe preservation of genetically transformed plant materials. Adv Genet Eng Biotechnol 1:1–2CrossRef
  Wang B, Wang RR, Cui ZH, Li JW, Bi WL, Li BQ, Ozudogru EA, Volk GM, Wang QC (2014a) Potential applications of cryobiotechnology to plant genetic transformation and pathogen eradication. Biotechnol Adv 32:583–595CrossRef PubMed
  Wang RR, Gao XX, Chen L, Huo LQ, Li MF, Wang QC (2014b) Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification. Sci Hortic 176:330–339CrossRef
  Ye X, Busov V, Zhao N, Meilan R, Lisa M, McDonnell LM, Heather D, Coleman HD, Shawn D, Mansfield SD, Feng CF, Li Y, Cheng ZM (2011) Transgenic populus trees for forest products, bioenergy, and functional genomics. Cri Rev Plant Sci 30:415–434CrossRef
  Zambryski P, Joos H, Genetello C, Leemans J, Montagu MV, Schell J (1983) Ti-plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J 2:2143–2150PubMedCentral PubMed
  Zhang Z, Skjeseth G, Elameen A, Haugslien S, Sivertsen A, Wang QC, Blystad DR (2015) Field performance evaluation and genetic integrity assessment in Argyranthemum maderense plants recovered from cryopreserved shoot tips. In Vitro Cell Dev Biol Plant 51:505–513CrossRef
  
• 作者单位：Jing-Wei Li (1)
  Hou-Hua Li (2)
  Ren-Rui Wang (1)
  Xiao-Xia Gao (1)
  Qiao-Chun Wang (1)
  
  1. State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of Horticultural Crops of Northwest China of Ministry of Agriculture of China, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  2. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Torenia fournieri, a popular potted flower crop, is frequently used as a model plant in genetic transformation studies, and transgenic plants expressing various foreign genes have been obtained in this plant. A safe and long-term conservation of the transgenic plants is necessary. In this study, shoot tips of transgenic plants of T. fournieri were successfully cryopreserved by droplet-vitrification. Growth patterns, and morphologies of leaves and flowers were identical between the plantlets recovered from cryopreservation and the in vitro stock cultures (control). No polymorphic bands and no alternations at ploidy level assessed by ISSR and FCM, respectively, were found in the transgenic plantlets recovered from cryopreservation in comparison with the in vitro stock cultures. The transgene Cry 1Ab analyzed by PCR was maintained, and its expression levels measured by qRT-PCR did not change in the cryo-derived transgenic plantlets. Therefore, cryopreservation can be considered a promising strategy for safe and long-term conservation of the transgenes. Keywords Cry 1Ab Droplet-vitrification cryopreservation Genetic integrity Morphology Shoot tips Torenia fournieri