Characterization of resistance to multiple fungicides in Botrytis cinerea populations from Asian ginseng in northeastern China

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• 作者：Xiao Hong Lu ; Xiao Lin Jiao ; Jianjun J. Hao…
• 关键词：Grey mold ; Multi ; fungicide resistance ; QoI fungicides ; Panax ginseng ; cyt b ; bos1
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：144
• 期：3
• 页码：467-476
• 全文大小：332 KB
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• 作者单位：Xiao Hong Lu (1)
  Xiao Lin Jiao (1)
  Jianjun J. Hao (2)
  Amanda Juan Chen (1)
  Wei Wei Gao (1)
  
  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China
  2. School of Food and Agriculture, The University of Maine, Orono, ME, 04469, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Botrytis cinerea infects ginseng throughout the whole growing season and results in significant economic losses. Fungicides have been the primary strategy in disease control, but frequent applications can increase the risk of fungicide resistance. To determine the resistance of field populations, 76 B. cinerea isolates were collected from 10 ginseng fields in Northeastern China in 2012 and 2013. The sensitivities of these isolates were examined to carbendazim, iprodione, pyrimethanil and azoxystrobin by analyzing mycelial growth inhibition. Almost all test isolates were resistant to at least one fungicide. For example, 27.6 %, 49.9 % and 21.1 % of the population was resistant to one, two or three of four test fungicides, respectively. There was only one isolate that was sensitive to all four fungicides. However, none were simultaneously resistant to all of the four fungicides. Mutations for E198A in the β-tubulin gene and G143A in the cyt b gene were found in all carbendazim- and azoxystrobin-resistant isolates, respectively, indicating a high level of resistance. Various mutations in the bos1 gene were associated with iprodione resistance, among which two combined point mutations, D757N and E369P, could be a novel genotype. Gene structure analysis showed that 50 % of the isolates carried the Bcbi143/144 intron in cyt b, indicating a high inherent risk for the development of G143A-associated resistance to quinone outside inhibitor fungicides. Most resistant isolates did not show reduced compound fitness in mycelial growth, sclerotial and conidial production, aggressiveness and sensitivity to osmotic stress. In conclusion, multi-fungicide resistance of B. cinerea contributed to the inefficiency of current fungicides. Keywords Grey mold Multi-fungicide resistance QoI fungicides Panax ginseng cyt b bos1