Analysis of murine B-cell epitopes on bluetongue virus 12 nonstructural protein 1

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• 作者：Wang HaiXiu (1)
  Sun EnCheng (1)
  Xu QingYuan (1)
  Yang Tao (1)
  Zhang Qin (1)
  Feng YuFei (1)
  Li JunPing (1)
  Lv Shuang (1)
  Sun Liang (1)
  Sun Jing (1)
  Wu DongLai (1)
  
• 关键词：Bluetongue virus ; NS1 protein ; MAbs ; PAbs ; Epitopes
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：99
• 期：3
• 页码：1309-1321
• 全文大小：3,559 KB
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• 作者单位：Wang HaiXiu (1)
  Sun EnCheng (1)
  Xu QingYuan (1)
  Yang Tao (1)
  Zhang Qin (1)
  Feng YuFei (1)
  Li JunPing (1)
  Lv Shuang (1)
  Sun Liang (1)
  Sun Jing (1)
  Wu DongLai (1)
  
  1. The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, 150001, Harbin, People鈥檚 Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The bluetongue virus (BTV) NS1 protein is one of the major proteins synthesized during BTV infection and is responsible for the generation of virus-specific tubules. Although some functional and structural studies on the BTV NS1 protein have been reported, there have been no reports describing the linear B-cell epitopes recognized by humoral immune responses published to date. In this study, 25 BTV12 NS1-reactive monoclonal antibodies (MAbs) and polyclonal antisera (polyclonal antibodies, PAbs) were generated and analyzed. We identified 14 linear NS1 epitopes recognized by the PAbs and MAbs using NS1-derived peptides in an enzyme-linked immunosorbent assay. Moreover, we predicted 23 linear B-cell epitopes using the ABCpred online server which employs an artificial neural network. Analysis of the predicted and identified epitopes of NS1 demonstrated the feasibility of B-cell epitope prediction. Sequence alignments indicated that the epitopes recognized by MAbs are highly conserved among BTV serotypes, but not among the other members of the genus Orbivirus, such as the African horse sickness virus (AHSV), epizootic hemorrhagic disease virus (EHDV), and Chuzan disease virus (CV). Importantly, we identified specific MAbs that recognized all BTV serotypes tested as well as MAbs that recognized only BTV12, suggesting that these NS1-specific MAbs could serve as a basis for BTV diagnostic approaches. The generation and identification of NS1 protein epitopes will provide the foundation for further studies about the function and structure of NS1 and novel epitope-based vaccines.