Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6

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• 作者：Kaifeng Wang ; Yue Wu ; Dan Yin ; Shixing Tang ; Guifang Hu ; Yaqing He
• 关键词：Recombinase polymerase amplification ; Hand ; foot and mouth disease ; Coxsackievirus A6
• 刊名：Archives of Virology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：162
• 期：1
• 页码：287-290
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Virology; Medical Microbiology; Infectious Diseases;
• 出版者：Springer Vienna
• ISSN：1432-8798
• 卷排序：162

文摘

Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.