Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

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• 作者：Baek-Rock Oh (1)
  Sun-Yeon Heo (1)
  Sung-Mok Lee (1)
  Won-Kyung Hong (1)
  Jang Min Park (1)
  You Ree Jung (1)
  Dae-Hyuk Kim (2)
  Jung-Hoon Sohn (3)
  Jeong-Woo Seo (1)
  Chul Ho Kim (1)
  
• 关键词：Crude glycerol ; Genetic engineering ; 2 ; Butanol/isobutanol ; Klebsiella pneumoniae
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：36
• 期：1
• 页码：57-62
• 全文大小：281 KB
• 作者单位：Baek-Rock Oh (1)
  Sun-Yeon Heo (1)
  Sung-Mok Lee (1)
  Won-Kyung Hong (1)
  Jang Min Park (1)
  You Ree Jung (1)
  Dae-Hyuk Kim (2)
  Jung-Hoon Sohn (3)
  Jeong-Woo Seo (1)
  Chul Ho Kim (1)
  
  1. Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea
  2. Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Jeonbuk, 561-756, South Korea
  3. Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejeon, 305-333, South Korea
  
• ISSN：1573-6776

文摘

Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ?em class="a-plus-plus">ldhA/pBR-iBO (ilvIH-em class="a-plus-plus">ilvC–ilvD–kivd–adhA), produced 2-butanol (160?mg?l?) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320?mg?l?. This represents the first successful attempt to produce 2-butanol from crude glycerol.