Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment

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• 作者：Sudha Joshi (1)
  Amanjot Kaur (1)
  Prince Sharma (2)
  Kusum Harjai (2)
  Neena Capalash (1)
  
• 关键词：Pseudomonas aeruginosa ; Virulence factors ; Quorum sensing ; AiiA lactonase ; Lactobacillus plantarum ; Delivery vehicle
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：30
• 期：8
• 页码：2241-2249
• 全文大小：730 KB
• 参考文献：1. Amiel E, Lovewell RR, O’Toole GA, Hogan DA, Berwin B (2010) / Pseudomonas / aeruginosa evasion of phagocytosis is mediated by loss of swimming motility and is independent of flagellum expression. Infect Immun 78:2937-945 CrossRef
  2. Anokhina IV, Kravtsov EG, Yashina NV, Ermolaev AV, Chesnokova VL, Dalin MV (2006) Surface adhesins of / lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media. Bull Exp Biol Med 142:609-13 CrossRef
  3. Aukrust T, Blom H (1992) Transformation of / Lactobacillus strains used in meat and vegetable fermentations. Food Res Int 25:253-61 CrossRef
  4. Chan RCY, Reid G, Irvinr T, Brucea W, Costerton JW (1985) Competitive exclusion of uropathogens from human uroepithelial cells by / Lactobacillus whole cells and cell wall fragment. Infect Immun 47:84-9
  5. Chen R, Zhou Z, Cao Y, Bai Y, Yao B (2010) High yield expression of an AHL-lactonase from / Bacillus sp. B546 in / Pichia / pastoris and its application to reduce / Aeromonas hydrophila mortality in aquaculture. Microb Cell Fact 9:39 CrossRef
  6. Cook DM, Li P-L, Ruchaud F, Padden S, Farrand SK (1997) Ti plasmid conjugation is independent of / vir: reconstitution of the / tra functions from pTiC58 as a binary system. J Bacteriol 179:1291-297
  7. Dekimpe V, Deziel E (2009) Revisiting the quorum-sensing hierarchy in / Pseudomonas aeruginosa the transcriptional regulator RhlR regulates LasR-specific factors. Microbiology 155:712-23 CrossRef
  8. del Rio B, Seegers JF, Gomes-Solecki M (2010) Immune response to / Lactobacillus plantarum expressing / Borrelia burgdorferi OspA is modulated by the lipid modification of the antigen. PLoS ONE 5(6):e11199 CrossRef
  9. Diep DB, Straume D, Kjos M, Torres C, Nes IF (2009) An overview of the mosaic bacteriocin / pln loci from / Lactobacillus plantarum. Peptides 30:1562-574 CrossRef
  10. Dover SE, Aroutcheva AA, Faro S, Chikindas ML (2008) Natural antimicrobials and their role in vaginal health: a short review. Int J Probiotics Prebiotics 3:219-30
  11. El Solh AA, Alhajhusain A (2009) Update on the treatment of / Pseudomonas aeruginosa pneumonia. J Antimicrob Chemother 64:229-38 CrossRef
  12. Falagas ME, Bliziotis IA, Kasiakou SK, Samonis G, Athanassopoulou P, Michalopoulos A (2005) Outcome of infections due to pan-drug-resistant (PDR) Gram-negative bacteria. BMC Infect Dis 5:24 CrossRef
  13. Heiniger RW, Winther-Larsen HC, Pickles RJ, Koomey M, Wolfgang MC (2010) Infection of human mucosal tissue by / Pseudomonas aeruginosa requires sequential and mutually dependent virulence factors and a novel pilus-associated adhesin. Cell Microbiol 12:1158-173 CrossRef
  14. Hendrickson EL, Plotnikova J, Mahajan-Miklos S, Rahme LG, Ausubel FM (2001) Differential roles of the / Pseudomonas aeruginosa PA14 / rpoN gene in pathogenicity in plants, nematodes, insects and mice. J Bacteriol 183:7126-134 CrossRef
  15. Hentzer M, Givskov M (2003) Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clin Invest 112:1300-307 CrossRef
  16. Iglewski BH, Kabat D (1975) NAD-independent inhibition of protein synthesis by / Pseudomonas aeruginosa toxin. Proc Natl Acad Sci USA 72:2284-288 CrossRef
  17. Martinez-Medina M, Naves P, Blanco J, Aldeguer X, Blanco JE, Blanco M, Ponte C, Soriano F, Darfeuille-Michaud A, Garcia-Gil LJ (2009) Biofilm formation as a novel phenotypic feature of adherent-invasive / Escherichia coli (AIEC). BMC Microbiol 9:202 CrossRef
  18. Matsumoto T, Ishikawa H, Tateda K, Yaeshima T, Ishibashi N, Yamaguchi K (2008) Oral administration of / Bifidobacterium / longum prevents gut-derived / Pseudomonas aeruginosa sepsis in mice. J Appl Microbiol 104:672-80 CrossRef
  19. McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, Daykin M, Lamb JH, Swift S, Bycroft BW, Stewart GSAB, Williams P (1997) Quorum sensing and / Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143:3703-711 CrossRef
  20. McClure CD, Schiller NL (1992) Effects of / Pseudomonas aeruginosa rhamnolipids on human monocyte-derived macrophages. J Leukoc Biol 51:97-02
  21. Nicodème M, Grill JP, Humbert G, Gaillard JL (2005) Extracellular protease activity of different / Pseudomonas strains: dependence of proteolytic activity on culture conditions. J Appl Microbiol 99:641-48 CrossRef
  22. Niu C, Clemmer KM, Bonomo RA, Rather PN (2008) Isolation and characterization of an autoinducer synthase from / Acinetobacter baumannii. J Bacteriol 190:3386-392 CrossRef
  23. O’Toole GA (2011) Microtiter dish biofilm formation assay. J Vis Exp 47:e2437
  24. O’sullivan DJ, Klaenhammer TR (1993) Rapid mini-prep isolation of high-quality plasmid DNA from / Lactococcus and / Lactobacillus spp. Appl Environ Microbiol 59:2730-733
  25. Panikov NS, Nikolaev YA (2002) Growth and adhesion of / Pseudomonas fluorescens in a batch culture: a kinetic analysis of the action of extracellular antiadhesins. Mikrobiologiia 71:619-28
  26. Papagianni M, Avramidis N, Filioussis G (2007) High efficiency electrotransformation of / Lactococcus lactis spp. Lactis cells pretreated with lithium acetate and dithiothreitol. BMC Biotechnol 7:15-0 CrossRef
  27. Park SC, Hwang MH, Kim YH, Kim JC, Song JC, Lee KW, Jeong KS, Rhee MH, Kim KS, Kim TW (2006) Comparison of pH and bile resistance of / Lactobacillus acidophilus strains isolated from rat, pig, chicken, and human sources. World J Microbiol Biotechnol 22:35-7 CrossRef
  28. Peral MC, Martinez MA, Valdez JC (2009) Bacteriotherapy with / Lactobacillus plantarum in burns. Int Wound J 6:73-1 CrossRef
  29. Reid G, Bruce AW (2006) Probiotics to prevent urinary tract infections: the rationale and evidence. World J Urol 24:28-2 CrossRef
  30. Reid G, Burton J (2002) Use of / Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect 4:319-24 CrossRef
  31. Reimmann C, Ginet N, Michel L, Keel C, Michaux P, Krishnapillai V, Zala M, Heurlier K, Triandafillu K, Harms H, Defago G, Haas D (2002) Genetically programmed autoinducer destruction reduces virulence gene expression and swarming motility in / Pseudomonas aeruginosa PAO1. Microbiology 148:923-32
  32. Sambrook J, Russell D (eds) (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  33. Schipper C, Hornung C, Bijtenhoorn P, Quitschau M, Grond S, Streit WR (2009) Metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in / Pseudomonas aeruginosa. Appl Environ Microbiol 75:224-33 CrossRef
  34. Sorvig E, Mathiesen G, Naterstad K, Eijsink VGH, Axelsson L (2005) High-level, inducible gene expression in / Lactobacillus sakei and / Lactobacillus plantarum using versatile expression vectors. Microbiology 151:2439-446 CrossRef
  35. Su HC, Ramkissoon K, Doolittle J, Clark M, Khatun J, Secrest A, Wolfgang MC, Giddings MC (2010) The development of ciprofloxacin resistance in / Pseudomonas aeruginosa involves multiple response stages and multiple proteins. Antimicrob Agents Chemother 54:4626-635 CrossRef
  36. Tevi-Bénissan C, Bélec L, Lévy M, Schneider-Fauveau V, Mohamed AS, Hallouin MC, Matta M, Grésenguet G (1997) In vivo semen-associated pH neutralization of cervicovaginal secretions. Clin Diagn Lab Immunol 4:367-74
  37. Thomas PW, Stone EM, Costello AL, Tierney DL, Fast W (2005) The quorum-quenching lactonase from / Bacillus thuringiensis is a metalloprotein. Biochemistry 44:7559-569 CrossRef
  38. Ulrich RL (2004) Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in / Burkholderia thailandensis. Appl Environ Microbiol 70:6173-180 CrossRef
  39. Velraeds MC, van der Mei HC, Reid G, Busscher HJ (1996) Inhibition of initial adhesion of uropathogenic / Enterococcus faecalis by biosurfactants from / Lactobacillus isolates. Appl Environ Microbiol 62:1958-963
  40. Venturi V (2006) Regulation of quorum sensing in / Pseudomonas. FEMS Microbiol Rev 30:274-91 CrossRef
  41. Visca P, Chiarini F, Mansi A, Vetriani C, Serino L, Orsi N (1992) Virulence determinants in / Pseudomonas aeruginosa strains from urinary tract infections. Epidemiol Infect 108:323-36 CrossRef
  42. Vogelmann SA, Seitter M, Singer U, Brandt MJ, Hertel C (2009) Adaptability of lactic acid bacteria and yeasts to sourdoughs prepared from cereals, pseudocereals and cassava and use of competitive strains as starters. Int J Food Microbiol 130:205-12 CrossRef
  43. Wargo MJ, Hogan DA (2007) Examination of / Pseudomonas aeruginosa / lasI regulation and 3-oxo-C12-homoserine lactone production using a heterologous / Escherichia coli system. FEMS Microbiol Lett 273:38-4 CrossRef
  44. Wells JM, Mercenier A (2008) Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat Rev Microbiol 6:349-62 CrossRef
  45. Wouafo M, Nzouankeu A, Kinfack JA, Fonkoua MC, Ejenguele G, Njine T, Ngandjio A (2010) Prevalence and antimicrobial resistance of / Salmonella serotypes in chickens from retail markets in Yaounde (Cameroon). Microb Drug Resist 16:171-76 CrossRef
  46. Yates EA, Philipp B, Buckley C, Atkinson S, Chhabra SR, Sockett RE, Goldner M, Dessaux Y, Camara M, Smith H, Williams P (2002) N-acylhomoserine lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of / Yersinia pseudotuberculosis and / Pseudomonas aeruginosa. Infect Immun 70:5635-645 CrossRef
  
• 作者单位：Sudha Joshi (1)
  Amanjot Kaur (1)
  Prince Sharma (2)
  Kusum Harjai (2)
  Neena Capalash (1)
  
  1. Department of Biotechnology, Panjab University, Chandigarh, 160014, India
  2. Department of Microbiology, Panjab University, Chandigarh, 160014, India
  
• ISSN：1573-0972

文摘

Pseudomonas aeruginosa possesses an arcade of both cell-associated and extracellular cytotoxic virulence factors which are regulated by a multi-component quorum sensing system. Many research studies report success of lactonase in combating the pathogenicity of P. aeruginosa but delivery of lactonase remains a challenge. The present study aims at developing a delivery vehicle for lactonase. Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector. pSIP409: aiiA construct was stably maintained in L. plantarum NC8. Co-culturing of multi-drug resistant (MDR) clinical isolates of P. aeruginosa and PAO1 with recombinant L. plantarum NC8 led to significant reduction (p?P. aeruginosa and also showed significant reduction in adhesion of P. aeruginosa strains to uroepithelial cells in vitro. This study shows the heterologous expression of AiiA lactonase in L. plantarum NC8. Co-culturing of lactonase expressing L. plantarum NC8 with MDR P. aeruginosa strains led to attenuation of their virulence significantly. These results underscore the potential application of recombinant L. plantarum NC8 with anti-quorum sensing properties to control infections caused by multidrug resistant P. aeruginosa.