A Lentiviral Vector Expressing Desired Gene Only in Transduced Cells: An Approach for Suicide Gene Therapy

详细信息    查看全文

• 作者：Zahra Mohammadi ; Laleh Shariati ; Hossein Khanahmad…
• 关键词：Lentivirus ; Suicide gene therapy ; Cancer
• 刊名：Molecular Biotechnology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：57
• 期：9
• 页码：793-800
• 全文大小：790 KB
• 参考文献：1.Escors, D., & Breckpot, K. (2010). Lentiviral vectors in gene therapy: their current status and future potential. Archivum Immunologiae et Therapiae Experimentalis, 58, 107-19.View Article
  2.Yazawa, K., Fisher, W. E., & Brunicardi, F. C. (2002). Current progress in suicide gene therapy for cancer. World Journal of Surgery, 26, 783-89.View Article
  3.Malecki, M. (2012). Frontiers in suicide gene therapy of cancer. Journal of Genetic Syndromes and Gene Therapy, 3, e114.View Article
  4.Salvatorelli, E., Menna, P., Cantalupo, E., Chello, M., Covino, E., Wolf, F. I., & Minotti, G. (2015). The concomitant management of cancer therapy and cardiac therapy. Biochimica et Biophysica Acta. doi:10.-016/?j.?bbamem.-015.-1.-03 .
  5.Springer, C. J., & Niculescu-Duvaz, I. (2000). Prodrug-activating systems in suicide gene therapy. The Journal of Clinical Investigation, 105, 1161-167.View Article
  6.Duarte, S., Carle, G., Faneca, H., de Lima, M. C., & Pierrefite-Carle, V. (2012). Suicide gene therapy in cancer: Where do we stand now? Cancer Letters, 324, 160-70.View Article
  7.Zarogoulidis, P., Darwiche, K., Sakkas, A., Yarmus, L., Huang, H., Li, Q., et al. (2013). Suicide gene therapy for cancer—current strategies. Journal of Genetic Syndromes & Gene Therapy, 4, 16849.
  8.Yang, W. S., Park, S. O., Yoon, A. R., Yoo, J. Y., Kim, M. K., Yun, C. O., & Kim, C. W. (2006). Suicide cancer gene therapy using pore-forming toxin, streptolysin O. Molecular Cancer Therapeutics, 5, 1610-619.View Article
  9.Chen, H. (2012). Exploiting the intron-splicing mechanism of insect cells to produce viral vectors harboring toxic genes for suicide gene therapy. Molecular Therapy—Nucleic Acids, 1, e57.View Article
  10.Massuda, E. S., Dunphy, E. J., Redman, R. A., Schreiber, J. J., Nauta, L. E., Barr, F. G., et al. (1997). Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proceedings of the National Academy of Sciences USA, 94, 14701-4706.View Article
  11.Keyvani, K., Baur, I., & Paulus, W. (1999). Tetracycline-controlled expression but not toxicity of an attenuated diphtheria toxin mutant. Life Sciences, 64, 17124.View Article
  12.Wang, C. Y., Li, F., Yang, Y., Guo, H. Y., Wu, C. X., & Wang, S. (2006). Recombinant baculovirus containing the diphtheria toxin A gene for malignant glioma therapy. Cancer Research, 66, 5798-806.View Article
  13.Kurayoshi, K., Ozono, E., Iwanaga, R., Bradford, A. P., Komori, H., & Ohtani, K. (2014). Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochemical and Biophysical Research Communications, 450, 240-46.View Article
  14.Li, Y., McCadden, J., Ferrer, F., Kruszewski, M., Carducci, M., Simons, J., & Rodriguez, R. (2002). Prostate-specific expression of the diphtheria toxin A chain (DT-A): Studies of inducibility and specificity of expression of prostate-specific antigen promoter-driven DT-A adenoviral-mediated gene transfer. Cancer Research, 62, 2576-582.
  15.Kohlschutter, J., Michelfelder, S., & Trepel, M. (2010). Novel cytotoxic vectors based on adeno-associated virus. Toxins (Basel), 2, 2754-768.View Article
  16.McTaggart, S., & Al-Rubeai, M. (2002). Retroviral vectors for human gene delivery. Biotechnology Advances, 20, 1-1.View Article
  17.Santhosh, C. V., Tamhane, M. C., Kamat, R. H., Patel, V. V., & Mukhopadhyaya, R. (2008). A lentiviral vector with novel multiple cloning sites: Stable transgene expression in vitro and in vivo. Biochemical and Biophysical Research Communications, 371, 546-50.View Article
  18.Freed, E. O. (2001). HIV-1 replication. Somatic Cell and Molecular Genetics, 26, 13-3.View Article
  19.Vecil, G. G., & Lang, F. F. (2003). Clinical trials of adenoviruses in brain tumors: A review of Ad-p53 and oncolytic adenoviruses. Journal of Neuro-oncology, 65, 237-46.View Article
  20.Sudarshan, S., Holman, D. H., Hyer, M. L., Voelkel-Johnson, C., Dong, J. Y., & Norris, J. S. (2005). In vitro efficacy of Fas ligand gene therapy for the treatment of bladder cancer. Cancer Gene Therapy, 12, 12-8.View Article
  21.Ozawa, T., Hu, J. L., Hu, L. J., Kong, E. L., Bollen, A. W., Lamborn, K. R., & Deen, D. F. (2005). Functionality of hypoxia-induced BAX expression in a human glioblastoma xenograft model. Cancer Gene Therapy, 12, 449-55.
  22.Chen, H. (2012). Exploiting the intron-splicing mechanism of insect cells to produce viral vectors harboring toxic genes for suicide gene therapy. Molecular Therapy—Nucleic Acids, 1, e57.View Article
  23.Maxwell, I. H., Maxwell, F., & Glode, L. M. (1986). Regulated expression of a diphtheria toxin A-chain gene transfected into human cells: possible strategy for inducing cancer cell suicide. Cancer Research, 46, 4660-664.
  24.Goverdhana, S., Puntel, M., Xiong, W., Zirger, J. M., Barcia, C., Curtin, J. F., et al. (2005
• 作者单位：Zahra Mohammadi (1)
  Laleh Shariati (2)
  Hossein Khanahmad (1) (3)
  Mahsa Kolahdouz (1)
  Fariborz Kianpoor (4)
  Jahan Afrooz Ghanbari (1)
  Zahra Hejazi (1)
  Mansoor Salehi (1)
  Parvaneh Nikpour (1) (3) (5)
  Mohammad Amin Tabatabaiefar (1) (3)
  
  1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran
  3. Child Growth and Development Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  5. Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  
• 刊物主题：Biotechnology; Biochemistry, general; Cell Biology; Protein Science; Biological Techniques; Human Genetics;
• 出版者：Springer US
• ISSN：1559-0305

文摘

Suicide gene therapy is a therapeutic strategy, in which cell suicide inducing transgenes are introduced into target cells. Inserting a toxin-encoding gene into a lentiviral vector leads to decreased efficiency of virus production due to lethal effect of toxin on packaging cells. In this study, we designed and constructed a transfer vector to express the toxin in transduced cells but not in packaging cells. Plasmid pLenti-F/GFP was constructed by cutting out R 5′LTR-R 3′LTR fragment with the AflII restriction endonuclease from a plasmid pLenti4-GW/H1/TO-laminshRNA, followed by ligating R 5′LTR-R 3′LTR fragment, constructed by three PCR stages. The promoter and GFP CDS were inserted in opposite strand. For lentiviral production, the HEK293T cell line was co-transfected with the PMD2G, psPAX2, and pLenti-F/GFP plasmids (envelope, packaging, and transfer plasmids).Viral vector titers were assayed. The HEK293T cell line was transduced with this virus. PCR was performed to confirm the presence of the promoter fragment between the R and U5 in 3′LTR. The lentivirus titers were approximately 2?×?105. The GFP expression was seen in 51?% of the HEK293T cells transduced with lentivirus. The PCR product size was 1440?bp confirming the promoter fragment position between the R and U5 in 3′LTR. The strategy enables us to use a broad spectrum of toxin genes in gene therapy and helps avoid the death of the packaging cells with lentiviral vectors carrying a toxin-encoding gene, thereby increasing the efficiency of viral production in packaging cells.