Direct electron transfer of horseradish peroxidase at Co₃O₄–graphene nanocomposite modified electrode and electrocatalysis

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• 作者：Wen Zheng ; Wei Chen ; Wenju Weng ; Lihua Liu…
• 关键词：Co3O4–graphene nanocomposite ; Horseradish peroxidase ; Carbon ionic liquid electrode ; Direct electron transfer ; Electrocatalysis
• 刊名：Journal of the Iranian Chemical Society
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：14
• 期：4
• 页码：925-932
• 全文大小：
• 刊物主题：Analytical Chemistry; Inorganic Chemistry; Physical Chemistry; Biochemistry, general; Organic Chemistry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1735-2428
• 卷排序：14

文摘

In this paper, the mixture of Co₃O₄–graphene nanocomposite and horseradish peroxidase (HRP) was spread on the surface of carbon ionic liquid electrode (CILE). Then, Nafion film was used for the immobilization. The results of spectroscopy proved that HRP kept up its native structure in the complex material. Direct electrochemistry of HRP resulted in a couple of quasi-reversible redox waves on cyclic voltammograms, reflecting the realization of direct electron transfer of HRP with electrode. The improvement in electrochemical responses was due to the usage of highly conductive Co₃O₄–graphene nanocomposite with biocompatible interface. Electrochemical parameters such as the electron transfer coefficient (α) was estimated as 0.47, and the apparent heterogeneous electron transfer rate constant (k_s) was calculated as 2.90 s−1. The HRP modified electrode exhibited good electrochemical catalytic ability toward the reduction of trichloroacetic acid and NaNO₂. As a consequence, an updated third-generation electrochemical HRP biosensor with Co₃O₄–GR/CILE was constructed successfully.