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Regulation of vincamine biosynthesis and associated growth promoting effects through abiotic elicitation, cyclooxygenase inhibition, and precursor feeding of bioreactor grown Vinca minor hairy roots
- 作者:Priyanka Verma (1)
Shamshad Ahmad Khan (1) Ajay Kumar Mathur (1) Karuna Shanker (2) Raj Kishori Lal (3)
- 关键词:Vinca minor ; Vincamine ; Hairy roots ; Bioreactor up ; scaling ; Indole alkaloid regulation
- 刊名:Applied Biochemistry and Biotechnology
- 出版年:2014
- 出版时间:June 2014
- 年:2014
- 卷:173
- 期:3
- 页码:663-672
- 全文大小:
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- 作者单位:Priyanka Verma (1)
Shamshad Ahmad Khan (1) Ajay Kumar Mathur (1) Karuna Shanker (2) Raj Kishori Lal (3)
1. Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Kukrail Picnic Spot Road, Lucknow, 226015, India 2. Department of Analytical Chemistry, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Lucknow, 226015, India 3. Department of Plant Breeding and Genetics, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Lucknow, 226015, India
- ISSN:1559-0291
文摘
Hydroxylase/acetyltransferase elicitors and cyclooxygenase inhibitor along with various precursors from primary shikimate and secoiridoid pools have been fortified to vincamine less hairy root clone of Vinca minor to determine the regulatory factors associated with vincamine biosynthesis. Growth kinetic studies revealed that acetyltransferase elicitor acetic anhydride and terpenoid precursor loganin significantly reduce the growth either supplemented alone or in combination (GI--40.6?±-8.5 to 246.7?±-4.3), while shikimate and tryptophan trigger biomass accumulation (GI--40.2?±-1.5 to 540.5?±-0.3). Loganin also downregulates total alkaloid biosynthesis. Maximum flux towards vincamine production (0.017?±-.001?% dry wt.) was obtained when 20-day-old hairy roots were fortified with secologanin (10?mg/l) along with tryptophan (100?mg/l), naproxen (8.4?mg/l), hydrogen peroxide (20?μg/l), and acetic anhydride (32.4?mg/l). This was supported by RT PCR (qPCR) analysis where 2- and 3-fold increase in tryptophan decarboxylase (TDC; RQ--.0?±-.09) and strictosidine synthase (STR; RQ--.3?±-.36) activity, respectively, was recorded. The analysis of variance (ANOVA) for growth kinetics, total alkaloid content, and gene expression studies favored highly significant data (P-lt;-.05-.01). Above treated hairy roots were also up-scaled in a 5-l stirred-tank bioreactor where a 40-day cycle yielded 8-fold increase in fresh root mass.
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