文摘
In Vitis labruscana ‘Pione’ and ‘Kyoho’, 57 grapevines with rugose wood (RW) symptoms and 31 asymptomatic grapevines were obtained from vineyards in Hiroshima and Okayama prefectures. We surveyed vines for RW-associated viruses and other viruses by reverse transcription-polymerase chain reaction with virus-specific primers and degenerate primers for simultaneous detection of viruses associated with RW or leafroll disease. Rupestris stem pitting-associated virus (RSPaV) was detected from all grapevines with RW symptoms and from eight asymptomatic grapevines. Grapevine leafroll-associated virus-1, -2, -3, Grapevine virus B, Grapevine fleck virus, and Grapevine asteroid mosaic-associated virus were detected from some of the grapevines tested. RSPaV isolates detected were clustered in four groups by phylogenetic analysis using the nucleotide sequences of coat protein gene. These results suggest that RSPaV with diverse genetic variants is highly related to RW in Japanese grapevine cultivars Pione and Kyoho.
