A non-fluidic, fluorometric assay for the detection of fungi on cultural heritage materials

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• 作者：Nick R. Konkol (1)
  Archana Vasanthakumar (2)
  Alice DeAraujo (2)
  Ralph Mitchell (2)
  
• 关键词：Fungi ; Conidia ; Non ; fluidic ; Early detection ; Cultural heritage
• 刊名：Annals of Microbiology
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：63
• 期：3
• 页码：965-970
• 全文大小：282KB
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• 作者单位：Nick R. Konkol (1)
  Archana Vasanthakumar (2)
  Alice DeAraujo (2)
  Ralph Mitchell (2)
  
  1. Department of Biology, Indiana University Bloomington, Jordan Hall, 1001 East Third Street, Bloomington, IN, 47405-3700, USA
  2. Laboratory of Applied Microbiology, School of Engineering and Applied Sciences, Harvard University, 58 Oxford St, Cambridge, MA, 02138, USA
  
• ISSN：1869-2044

文摘

Cultural heritage materials are particularly susceptible to biodeterioration by fungi. Improper care and storage of artifacts contaminated with fungal material can promote the growth of these microscopic organisms and the inevitable deterioration that follows. Technology capable of detecting vegetative fungi and their reproductive structures could facilitate the struggle against fungal biodeterioration. Archivists and conservators could be notified of fungal contamination within a collection and apply pre-emptive measures, such as modification of environmental conditions, to prevent biodeterioration. The aim of this study was to improve and simplify a fluorometric assay used for the early detection of minute quantities of fungal biomass on cultural heritage materials. To this end we have successfully developed a non-fluidic assay in which fluid transfers, centrifugation steps, and much of the specialized equipment formerly needed to perform the assay are eliminated. The time required for completion of the assay was reduced to 30?min. Use of the assay was also expanded to include the early detection of viable fungal conidia from several species of fungi. These refinements will expedite implementation of this technology by archivists and conservators as they monitor and combat the fungal deterioration of cultural heritage materials.