Identification of neurons that express ghrelin receptors in autonomic pathways originating from the spinal cord

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• 作者：John B. Furness (1) j.furness@unimelb.edu.au
  Hyun-Jung Cho (1)
  Billie Hunne (1)
  Haruko Hirayama (1)
  Brid P. Callaghan (1)
  Alan E. Lomax (2)
  James A. Brock (1)
• 关键词：Autonomic ganglia &#8211 ; Ghrelin &#8211 ; Immunohistochemistry &#8211 ; Preganglionic neurons &#8211 ; Retrograde tracing &#8211 ; Mouse
• 刊名：Cell and Tissue Research
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：348
• 期：3
• 页码：397-405
• 全文大小：1.0 MB
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• 作者单位：1. Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria 3010, Australia2. Gastrointestinal Diseases Research Unit, Queen鈥檚 University, Kingston, Ontario, Canada
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

Functional studies have shown that subsets of autonomic preganglionic neurons respond to ghrelin and ghrelin mimetics and in situ hybridisation has revealed receptor gene expression in the cell bodies of some preganglionic neurons. Our present goal has been to determine which preganglionic neurons express ghrelin receptors by using mice expressing enhanced green fluorescent protein (EGFP) under the control of the promoter for the ghrelin receptor (also called growth hormone secretagogue receptor). The retrograde tracer Fast Blue was injected into target organs of reporter mice under anaesthesia to identify specific functional subsets of postganglionic sympathetic neurons. Cryo-sections were immunohistochemically stained by using anti-EGFP and antibodies to neuronal markers. EGFP was detected in nerve terminal varicosities in all sympathetic chain, prevertebral and pelvic ganglia and in the adrenal medulla. Non-varicose fibres associated with the ganglia were also immunoreactive. No postganglionic cell bodies contained EGFP. In sympathetic chain ganglia, most neurons were surrounded by EGFP-positive terminals. In the stellate ganglion, neurons with choline acetyltransferase immunoreactivity, some being sudomotor neurons, lacked surrounding ghrelin-receptor-expressing terminals, although these terminals were found around other neurons. In the superior cervical ganglion, the ghrelin receptor terminals innervated subgroups of neurons including neuropeptide Y (NPY)-immunoreactive neurons that projected to the anterior chamber of the eye. However, large NPY-negative neurons projecting to the acini of the submaxillary gland were not innervated by EGFP-positive varicosities. In the celiaco-superior mesenteric ganglion, almost all neurons were surrounded by positive terminals but the VIP-immunoreactive terminals of intestinofugal neurons were EGFP-negative. The pelvic ganglia contained groups of neurons without ghrelin receptor terminal innervation and other groups with positive terminals around them. Ghrelin receptors are therefore expressed by subgroups of preganglionic neurons, including those of vasoconstrictor pathways and of pathways controlling gut function, but are absent from some other neurons, including those innervating sweat glands and the secretomotor neurons that supply the submaxillary salivary glands.