Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes

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• 作者：Minakshi Choudhary ; Jayanand…
• 关键词：Drought stress ; ESTs ; Pearl millet ; qRT ; PCR ; Stress responsive genes
• 刊名：Physiology and Molecular Biology of Plants
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：21
• 期：2
• 页码：187-196
• 全文大小：1,932 KB
• 参考文献：Allen RG, Tresini M (2000) Oxidative stress and gene regulation. Free Radic Biol Med 28:463-99View Article PubMed
  Almeida CMA, Donato MTS, Amaral DOJ, Lima GSA, Brito GG, Lima MMA, Correia MTS, Silva MV (2013) Differential gene expression in sugarcane induced by salicylic acid and under water deficit conditions. Agric Sci Res 3(1):38-4
  Altschul SF, Gish W, Miller W, Myers EW, Lipman D (1990) Basic local alignment search tool. J Mol Biol 215:403-10View Article PubMed
  Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413-28
  Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias AA (2008) The enigmatic LEA proteins and other hydrophilins. Plant Physiol 148:6-4View Article PubMed Central PubMed
  Bezan?on G, Pham JL, Deu M, Vigouroux Y, Sagnard F, Mariac C, Kapran I, Mamadou A, Gerard B, Ndjeunga J (2009) Changes in the diversity and geographic distribution of cultivated millet (Pennisetum glaucum [L.] R. Br.) and sorghum (Sorghum bicolor (L.) Moench) varieties in Niger between 1976 and 2003. Genet Resour Crop Evol 56:223-36View Article
  Caramelo JJ, Iusem ND (2009) When cells lose water: lessons from biophysics and molecular biology. Prog Biophys Mol Biol 99(1):1-View Article PubMed
  Caverzan A, Passaia G, Rosa SB, Rebeiro CW, Lazzarotto F, Margis-Pinheiro M (2012) Plant responses to stresses: role of ascorbate peroxidise in antioxidant protection. Genet Mol Biol 35(4):1011-019View Article PubMed Central PubMed
  Chang YC, Ma JF, Matsumoto H (1998) Mechanisms of Al-induced iron chlorosis in wheat (Triticum aestivum): al-inhibited biosynthesis and secretion of phytosiderophore. Physiol Plant 102:9-5View Article
  Chauhan H, Khurana N, Tayagi AK, Khurana JP, Khurana P (2011) Characterization of high temperature stress responsive gene in bread wheat (Tritcum aestivum L.) and their various stages of development. Plant Mol Biol 75:35-1View Article PubMed
  Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidiniumthiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-59View Article PubMed
  Covitz PA, Smith LS, Long SR (1998) Expressed sequence tags from a root-hair-enriched Medicago truncatula cDNA Library. Plant Physiol 117(4):1325-332View Article PubMed Central PubMed
  Czechowski T, Stitt M, Altmann T, Udvardi M, Scheible WR (2005) Genomewide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139:5-7View Article PubMed Central PubMed
  Devanathan S, Erban A, Perez-Torres R, Kopka J, Christopher A, Makaroff CA (2014) Arabidopsis thaliana Glyoxalase 2- is required during abiotic stress but is not essential under normal plant growth. PLoS ONE 9(4):1-2View Article
  Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci U S A 93:6025-030View Article PubMed Central PubMed
  Ding H, Zhang ZM, Qin FF, Dai LX, Li CJ, Ci DW, Song WW (2014) Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization. Electron J Biotechnol 7(6):304-10View Article
  Dominguez F, Cejudo FJ (1996) Characterization of the endoproteases appearing during wheat grain development. Plant Physiol 112:1211-217PubMed Central PubMed
  Fatima S, Arshad M, Chaudhari SK, Ali A, Amjad MS, Kausar R (2014) Utilization of synthetics for drought tolerance in bread wheat (Triticum aestivum L.). International J Bioscience 5(1):104-12
  Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239-47View Article PubMed
  Frankel N, Hasson E, Iusem ND, Rossi MS (2003) Adaptive evolution of the water stress-induced gene Asr2 in Lycopersicon species dwelling in arid habitats. Mol Biol Evol 20:1955-962View Article PubMed
  Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot 60(12):3531-544View Article PubMed Central PubMed
  Hubank M, Schatz DG (1994) Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res 22(25):5640-648View Article PubMed Central PubMed
  ICAR- AICRP (All India Coordinated Research Project on Pearl Millet) (2014) Annual report 2013-014. http://?www.?aicrpmip.?res.?in
  Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real time PCR. Biochem Biophys Res Commun 345:646-51View Arti
• 作者单位：Minakshi Choudhary (1)
  Jayanand (2)
  Jasdeep Chatrath Padaria (1)
  
  1. Biotechnology and Climate Change Laboratory, National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012, India
  2. Shobhit University, NH-58, Modipuram, Meerut, 250110, India
  
• 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0430

文摘

Pearl millet (Pennisetum glaucum) is an important cereal of traditional farming systems that has the natural ability to withstand various abiotic stresses. The present study aims at the identification and validation of major differentially expressed genes in response to drought stress in P. glaucum by Suppression Subtractive Hybridization (SSH) analysis. Twenty-two days old seedlings of P. glaucum cultivar PPMI741 were subjected to drought stress by treatment of 30?% Polyethylene glycol for different time periods 30?min (T1), 2?h (T2), 4?h (T3), 8?h (T4), 16?h (T5), 24?h (T6) and 48?h (T7) respectively, monitored by examining the RWC of seedlings. Total RNA was isolated to construct drought responsive subtractive cDNA library through SSH, sequenced to identify the differentially expressed genes in response to drought stress and validated by qRT-PCR.745 ESTs were assembled into a collection of 299 unigenes having 52 contigs and 247 singletons. All 745 ESTs were submitted to ENA-EMBL databases (Accession no. HG516611- HG517355). After analysis, 10 differentially expressed genes were validated namely Abscisic stress ripening protein, Ascorbate peroxidase, Inosine-5-monophosphate dehydrogenase, Putative beta-1, 3-glucanase, Glyoxalase, Rab7, Aspartic proteinase Oryzasin, DnaJ—like protein and Calmodulin—like protein by qRT-PCR. The identified ESTs reveal a major portion of the stress responsive transcriptome that may prove to be a vent to unravel molecular basis underlying tolerance of pearl millet (Pennisetum glaucum) to drought stress. These genes could be utilized for transgenic breeding or transferred to crop plants through marker assisted selection for the development of better drought resistant cultivars having enhanced adaptability to survive harsh environmental conditions.