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Production of naphthoquinone derivatives using two-liquid-phase suspension cultures of Alkanna orientalis
- 作者:Sepideh Mahjouri ; Ali Movafeghi…
- 关键词:Alkanna orientalis ; Alkannin/shikonin derivatives ; Two ; liquid ; phase culture ; Liquid paraffin
- 刊名:Plant Cell, Tissue and Organ Culture
- 出版年:2016
- 出版时间:January 2016
- 年:2016
- 卷:124
- 期:1
- 页码:201-207
- 全文大小:727 KB
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- 作者单位:Sepideh Mahjouri (1)
Ali Movafeghi (1) Khadijeh Zare (1) Morteza Kosari-Nasab (2) Hossein Nazemiyeh (3)
1. Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran 2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 3. Research Center for Pharmaceutical Nanotechnology and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
- 刊物类别:Biomedical and Life Sciences
- 刊物主题:Life Sciences
Plant Sciences Plant Physiology
- 出版者:Springer Netherlands
- ISSN:1573-5044
文摘
An in vitro two-liquid-phase suspension culture system of Alkanna orientalis was established in order to evaluate the ability of cultured cells to produce naphthoquinone derivatives. Initially, callus tissues were prepared from cotyledon explants of A. orientalis on the solidified B5 medium supplemented with 1 mg/L 2,4-dicholorophenoxyacetic acid (2,4-D) and 2 mg/L kinetin (kin). Subsequently, fragile calli were transferred to liquid B5 medium with the same growth regulators for the initiation of cell suspension culture. Thereafter, the two-liquid-phase suspension culture was established using M9 medium and liquid paraffin as the absorbent. The presence of liquid paraffin efficiently induced the production of pigments by the cultured cells. The n-hexane extract of proliferated cell suspension culture was studied by analytical HPLC. Then, the main secondary metabolites were separated by preparative HPLC and their structures were elucidated by UV, 1H and 13C-NMR spectroscopy. Accordingly, alkannin/shikonin, alkannin/shikonin acetate, deoxyshikonofuran and a dimmer of alkannin/shikonin were identified as the main chemical compounds in the extract. On the basis of our results, the two-liquid-phase suspension culture led to the effective induction of naphthoquinone derivatives. These findings draw attention to the potential of A. orientalis cell suspension cultures in the production of naphthoquinone which may be considered as a source for the biosynthesis of these secondary metabolites for medicinal or industrial uses. Keywords Alkanna orientalis Alkannin/shikonin derivatives Two-liquid-phase culture Liquid paraffin
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