文摘
Neomycin is widely used in veterinary medicine, while the confirmatory analysis of neomycin residues in aquatic samples has not been well identified. Here, in this study, an immunoaffinity chromatography (IAC) for determination of neomycin residues in aquatic sample was developed and optimized. The average maximum reserved content of neomycin (MRC) was calculated as 1611 ng, and by the simple regeneration with phosphate buffer saline, the recovery value maintained above 75 % even at the 32nd reusage (at the loading amount of 1200 ng). With the combination of IAC and high-performance liquid chromatography (HPLC), a new strategy for the analysis of neomycin was proposed and validated with different aquatic products. With the spiking concentration from 400 to 1200 ng/Kg, the recovery value was observed in the range of 75.58–117.80 %, and the CV was lower than 11 %. All these results suggested that the IAC-HPLC is a new and promising technique for the routine monitoring of neomycin residue in aquatic products.
