Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury

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• 作者：Eyup Burak Sancak ; Hakan Turkön ; Selma Çukur ; Sevilay Erimsah…
• 关键词：major ozonated autohemotherapy ; renal ischemia ; reperfusion injury ; medical ozone ; neutrophil to lymphocyte ratio ; total oxidant status
• 刊名：Inflammation
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：39
• 期：1
• 页码：209-217
• 全文大小：1,201 KB
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• 作者单位：Eyup Burak Sancak (1) (7)
  Hakan Turkön (2)
  Selma Çukur (3)
  Sevilay Erimsah (4)
  Alpaslan Akbas (1)
  Murat Tolga Gulpinar (1)
  Huseyin Toman (5)
  Hasan Sahin (5)
  Metehan Uzun (6)
  
  1. Department of Urology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey
  7. Canakkale Onsekiz Mart Universitesi, Terzioglu Yerleskesi, Barbaros Mh, 17100, Canakkale, Turkey
  2. Department of Biochemistry, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey
  3. Department of Pathology, Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey
  4. Department of Histology and Embryology, Abant Izzet Baysal University, Faculty of Medicine, Bolu, Turkey
  5. Department of Anesthesiology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey
  6. Department of Physiology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

Medical ozone has therapeutic properties as an antimicrobial, anti-inflammatory, modulator of antioxidant defense system. Major ozonated autohemotherapy (MOA) is a new therapeutic approach that is widely used in the treatment of many diseases. The objective of the present study was to determine whether preischemic application of MOA would attenuate renal ischemia-reperfusion injury (IRI) in rabbits. Twenty-four male New Zealand white rabbits were divided into four groups, each including six animals: (1) Sham-operated group, (2) Ozone group (the MOA group without IRI), (3) IR group (60 min ischemia followed by 24 h reperfusion), and (4) IR + MOA group (MOA group). The effects of MOA were examined by use of hematologic and biochemical parameters consisting of neutrophil to lymphocyte ratio (NLR), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), ischemia-modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI). In addition, the histopathological changes including the tubular brush border loss (TBBL), tubular cast (TC), tubular necrosis (TN), intertubular hemorrhage and congestion (IHC), dilatation of bowman space (DBS), and interstitial inflammatory cells infiltration (IECI) were evaluated. In the IR group, compared to the Sham group, biochemical parameters indicating oxidative stress, NLR, IL-6, TNF-α, IMA, TOS, and OSI have increased. MOA reduced inflammation and oxidative stress parameters. Although TAS values have decreased in the IR group and increased in the MOA-pretreated group, no significant changes in TAS values were detected between the IR and MOA groups. The total score was obtained by summing all the scores from morphological kidney damage markers. The total score has increased with IR damage when compared with the Sham group (13.83 ± 4.30 vs 1.51 ± 1.71; p = 0.002). But, the total score has decreased significantly after application of MOA (5.01 ± 1.49; p = 0.002; compared with the IR group). MOA preconditioning is effective in reducing tissue damage induced in kidney ischemia-reperfusion injury. The protective effect of MOA is mediated via reducing inflammatory response and regulating of reactive oxygen species (ROS). Renal histology also showed convincing evidence regarding MOA’s protective nature against kidney injury induced renal ischemia-reperfusion. Consequently, MOA might be helpful in protecting the kidneys from IR-induced damage in humans, probably through the anti-inflammatory effect and reducing the total oxidant status. KEY WORDS major ozonated autohemotherapy renal ischemia-reperfusion injury medical ozone neutrophil to lymphocyte ratio total oxidant status