The Protective Effects of Melatonin Against Oxidative Stress and Inflammation Induced by Acute Cadmium Exposure in Mice Testis

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• 作者：Renyan Li ; Xue Luo ; Lianbing Li ; Qiang Peng…
• 关键词：Cadmium ; Melatonin ; Oxidative stress ; Inflammation ; Male reproduction
• 刊名：Biological Trace Element Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：170
• 期：1
• 页码：152-164
• 全文大小：2,427 KB
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• 作者单位：Renyan Li (2)
  Xue Luo (1)
  Lianbing Li (2)
  Qiang Peng (3)
  Yuyou Yang (2)
  Letian Zhao (2)
  Mingfu Ma (2)
  Zhiwei Hou (2)
  
  2. Chongqing Institute of Population and Family Planning, Key Laboratory of Birth Defects and Reproductive Health, Chongqing, 400020, People’s Republic of China
  1. Institute of Tropical Medicine, Third Military Medical University, Chongqing, 400038, People’s Republic of China
  3. Beibei District of Chongqing Municipal Public Security Bureau of Interpol Detachment, Chongqing, 400700, People’s Republic of China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

Cadmium (Cd) is widely used in daily life and was recently recognized as a possible source of human toxicity due to its ability to accumulate in organs. Previous studies have shown that Cd exposure may cause testicular toxicity through oxidative stress and an inflammatory effect. Melatonin has been demonstrated to be an effective anti-oxidant and has an anti-inflammatory effect. The aim of the present study was to investigate the toxicological effects of Cd on reproduction in male mice and the potential protective action of melatonin against these adverse effects. Adult male mice were injected intraperitoneally with Cd at a dose of 2 mg/kg body weight per day for seven consecutive days with or without melatonin pretreatment. Sex organ weight, sperm parameters including sperm quality, apoptosis, acrosome integrity, mitochondrial membrane potential, testicular morphology, serum sex hormone, inflammatory status, and oxidative stress were evaluated. The results showed that significant adverse effects were observed in the male reproductive system after Cd exposure, including alterations in sperm parameters, increased DNA damage, and sex hormone disturbance. Acute Cd exposure also significantly increased malondialdehyde (MDA) contents, decreased glutathione (GSH) and superoxide dismutase (SOD) activities, and upregulated levels of the pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α), and interleukin-1beta (IL-1β), in the testis. In contrast, melatonin pretreatment significantly alleviated these toxic effects, and its mechanism may involve inhibiting MDA level, restoring GSH and SOD activities, and reducing the upregulation of TNF-α and IL-1β. Our data suggest that oxidative stress and inflammation are involved in Cd-induced toxicity in the male reproductive system and that co-administration of melatonin exerts a protective effect against Cd-induced male reproductive toxicity. Keywords Cadmium Melatonin Oxidative stress Inflammation Male reproduction