Sesquiterpene lactone engineering in microbial and plant platforms: parthenolide and artemisinin as case studies

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• 作者：Mohammad Majdi ; Morahem Ashengroph…
• 关键词：Metabolic engineering ; Sesquiterpene lactones ; Cytochrome P450 ; Choloroplast ; Terpenes
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：100
• 期：3
• 页码：1041-1059
• 全文大小：977 KB
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• 作者单位：Mohammad Majdi (1) (2)
  Morahem Ashengroph (3)
  Mohammad Reza Abdollahi (4)
  
  1. Department of Agricultural Biotechnology, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
  2. Research Center for Medicinal Plant Breeding and Development, University of Kurdistan, Sanandaj, Iran
  3. Department of Biology and Biotechnology, Faculty of Sciences, University of Kurdistan, Sanandaj, Iran
  4. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Sesquiterpene lactones (SLs) are one of the most diverse groups of secondary metabolites that mainly have been observed in the Asteraceae. They are composed of a C₁₅ skeleton bearing functional groups, e.g., hydroxy, keto, or epoxy. Sesquiterpene lactones have been shown to display several biological activities; hence, their therapeutic effects are indispensable. To overcome low yield of sesquiterpene lactone content in native plants, manipulation of their biosynthetic pathway(s) has become an interesting approach for many researchers. Several genetic engineering strategies have been used in plants or microbial systems for elucidation of the biosynthetic pathway and high-level production of sesquiterpene lactones. Here, we will introduce ongoing research and perspectives about the manipulation of sesquiterpene lactone biosynthesis by various non-traditional metabolic engineering strategies, along with successful examples of high-yield production of sesquiterpene lactones mainly focused on parthenolide and artemisinin in plants and microorganisms.