Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners
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  • 作者：Mengru Zhang ; Ming Gong ; Yumei Yang ; Xujuan Li ; Haibo Wang…
  • 关键词：Ascorbate peroxidase 1 ; Fusion partner ; Heat ; susceptibility ; Hyper ; acidity ; Jatropha curcas ; Thermostability
  • 刊名：Biotechnology Letters
  • 出版年：2015
  • 出版时间：April 2015
  • 年：2015
  • 卷：37
  • 期：4
  • 页码：891-898
  • 全文大小：1,127 KB
  • 参考文献：1. Ahmad, P, Jaleel, CA, Salem, MA, Nabi, G, Sharma, S (2010) Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit Rev Biotechnol 30: pp. 161-175 CrossRef
    2. Chen, GX, Asada, K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30: pp. 987-998
    3. Davletova, S, Rizhsky, L, Liang, H, Shengqiang, Z, Oliver, DJ, Coutu, J, Shulaev, V, Schlauch, K, Mittler, R (2005) Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17: pp. 268-281 CrossRef
    4. Diaz-Vivancos, P, Faize, M, Barba-Espin, G, Faize, L, Petri, C, Hernández, JA, Burgos, L (2013) Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums. Plant Biotechnol J 11: pp. 976-985 CrossRef
    5. Faize, M, Burgos, L, Faize, L, Piqueras, A, Nicolas, E, Barba-Espin, G, Clemente-Moreno, MJ, Alcobendas, R, Artlip, T, Hernandez, JA (2011) Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress. J Exp Bot 62: pp. 2599-2613 CrossRef
    6. Huang, H, Liu, J, Marco, A (2006) Induced fit of passenger proteins fused to Archaea maltose binding proteins. Biochem Biophys Res Commun 344: pp. 25-29 CrossRef
    7. Li XJ (2013) Studies on improving the thermal stability of the rubisco activase of higher plants. Yunnan Normal University, Master thesis
    8. Lim, S, Kim, YH, Kim, SH, Kwon, SY, Lee, HS, Kim, JS, Cho, KY, Paek, KY, Kwak, SS (2007) Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling. Mol Breed 19: pp. 227-239 CrossRef
    9. Luke, JM, Carnes, AE, Sun, P, Hodgson, CP, Waugh, DS, Williams, JA (2011) Thermostable tag (TST) protein expression system: engineering thermotolerant recombinant proteins and vaccines. J Biotechnol 151: pp. 242-250 CrossRef
    10. Martell, JD, Deerinck, TJ, Sancak, Y, Poulos, TL, Mootha, VK, Sosinsky, GE, Ellisman, MH, Ting, AY (2012) Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Nat Biotechnol 30: pp. 1143-1148 CrossRef
    11. Mittler, R, Zilinskas, A (1993) Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Anal Biochem 212: pp. 540-546 CrossRef
    12. Panchuk, II, Volkov, RA, Sch?ffl, F (2002) Heat stress- and heat shock transcription factor- dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiol 29: pp. 838-853 CrossRef
    13. Park, SM, Jung, HY, Chung, KC, Rhim, H, Park, JH, Kim, J (2002) Stress-induced aggregation profiles of GST-alpha-synuclein fusion proteins: role of the C-terminal acidic tail of alpha-synuclein in protein thermosolubility and stability. Biochemistry 41: pp. 4137-4146 CrossRef
    14. Park, SM, Ahn, KJ, Jung, HY, Park, JH, Kim, J (2004) Effects of novel peptides derived from the acidic tail of synuclein (ATS) on the aggregation and stability of fusion proteins. Protein Eng Des Sel 17: pp. 251-260 CrossRef
    15. Pnueli, L, Liang, H, Rozenberg, M, Mittler, R (2003) Growth suppression, altered stomatal responses, and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants. Plant J 34: pp. 187-203
文摘
Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the level of plant cellular reactive oxygen species and its thermolability is proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion partners, such as the C-terminal peptide tails, were evaluated for their effects on the thermal stability and activity of APX1 from Jatropha curcas and Arabidopsis. The hyper-acidic fusion partners efficiently improved the thermostability and prevented thermal inactivation of APX1 in both plant species with an elevated heat tolerance of at least 2?°C. These hyper-acidified thermostable APX1 fusion variants are of considerable biotechnological potential and can provide a new route to enhance the heat tolerance of plant species especially of inherent thermo-sensitivity.
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